The study was designed to evaluate in vitro the cellular mechanisms of the single nucleotide polymorphism (SNP) p.N680S of the FSH receptor gene (FSHR) in human granulosa cells (GC) and included patients homozygous for the FSHR SNP (NN/SS) undergoing ovarian stimulation. GC were isolated during oocyte retrieval and cultured for 1–7 days. Basal oestradiol and progesterone concentrations were measured after short-term culture. The kinetics of cAMP, oestradiol and progesterone concentrations in response to various amounts of FSH were analysed in a 6–7 day culture. Basal oestradiol, but not progesterone, concentrations on day 1 of GC culture, were significantly higher in NN compared with SS (P = 0.045), but non-responsive to FSH stimulation. Immunofluorescence microscopy demonstrated the re-appearance of FSHR expression with increasing days in culture. Upon stimulation with FSH, GC cultured for 6–7 days displayed a dose-dependent increase of cAMP, oestradiol and progesterone but no difference in the EC50 values between both variants. Primary long-term GC cultures are a suitable system to study the effects of FSH in vitro. However, the experiments suggest that factors down-stream of progesterone production or external to GC might be involved in the clinically observed differences in an FSHR variant-mediated response to FSH

Effects of the FSH receptor gene polymorphism p.N680S on cAMP and steroid production in cultured primary human granulosa cells / V., Nordhoff; B., Sonntag; D., von Tils; M., Götte; Schüring, A. N.; J., Gromoll; K., Redmann; Casarini, Livio; Simoni, Manuela. - In: REPRODUCTIVE BIOMEDICINE ONLINE. - ISSN 1472-6483. - ELETTRONICO. - 23(2011), pp. 196-203. [10.1016/j.rbmo.2011.04.009]

Effects of the FSH receptor gene polymorphism p.N680S on cAMP and steroid production in cultured primary human granulosa cells.

CASARINI, Livio;SIMONI, Manuela
2011

Abstract

The study was designed to evaluate in vitro the cellular mechanisms of the single nucleotide polymorphism (SNP) p.N680S of the FSH receptor gene (FSHR) in human granulosa cells (GC) and included patients homozygous for the FSHR SNP (NN/SS) undergoing ovarian stimulation. GC were isolated during oocyte retrieval and cultured for 1–7 days. Basal oestradiol and progesterone concentrations were measured after short-term culture. The kinetics of cAMP, oestradiol and progesterone concentrations in response to various amounts of FSH were analysed in a 6–7 day culture. Basal oestradiol, but not progesterone, concentrations on day 1 of GC culture, were significantly higher in NN compared with SS (P = 0.045), but non-responsive to FSH stimulation. Immunofluorescence microscopy demonstrated the re-appearance of FSHR expression with increasing days in culture. Upon stimulation with FSH, GC cultured for 6–7 days displayed a dose-dependent increase of cAMP, oestradiol and progesterone but no difference in the EC50 values between both variants. Primary long-term GC cultures are a suitable system to study the effects of FSH in vitro. However, the experiments suggest that factors down-stream of progesterone production or external to GC might be involved in the clinically observed differences in an FSHR variant-mediated response to FSH
23
196
203
Effects of the FSH receptor gene polymorphism p.N680S on cAMP and steroid production in cultured primary human granulosa cells / V., Nordhoff; B., Sonntag; D., von Tils; M., Götte; Schüring, A. N.; J., Gromoll; K., Redmann; Casarini, Livio; Simoni, Manuela. - In: REPRODUCTIVE BIOMEDICINE ONLINE. - ISSN 1472-6483. - ELETTRONICO. - 23(2011), pp. 196-203. [10.1016/j.rbmo.2011.04.009]
V., Nordhoff; B., Sonntag; D., von Tils; M., Götte; Schüring, A. N.; J., Gromoll; K., Redmann; Casarini, Livio; Simoni, Manuela
File in questo prodotto:
File Dimensione Formato  
134.pdf

non disponibili

Descrizione: Articolo principale
Tipologia: Versione dell'editore (versione pubblicata)
Dimensione 623.64 kB
Formato Adobe PDF
623.64 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/699123
Citazioni
  • ???jsp.display-item.citation.pmc??? 20
  • Scopus 54
  • ???jsp.display-item.citation.isi??? 51
social impact