The sequence-specific amplification polymorphism (S-SAP) method,derived from the amplified fragment length polymorphism (AFLP)technique, produces amplified fragments containing retrotransposonlong terminal repeat (LTR) sequence at one end and a host restrictionsite at the other. The development and application of this procedure tothe LTR of the Vine-1 element from grapevine is reported. Twoprimers derived from one of the LTR sequences flanking theretrotransposon were used in combination with MseI degeneratedprimers on 15 grapevine accessions. S-SAP results were compared withAFLP data. The heterozygosity and gene diversity values were higherfor S-SAP than for the AFLP procedure. Results show that S-SAPamplification is effective in identifying polymorphisms and defininggenetic distances among cultivars, and could be used for fingerprintingand for Traminer clone identification. To the contrary Vine-1retrotransposon-based S-SAP was not able to distinguish Pinotclones.
Vine-1 retrotransposon-based S-SAP (sequence-specific amplified polymorphism) for Vitis vinifera L genotyping / Labra, M; Imazio, Serena Anna; Grassi, F; Rossoni, M; Sala, F.. - In: PLANT BREEDING. - ISSN 0179-9541. - ELETTRONICO. - 123:2(2004), pp. 180-185. [10.1046/j.1439-0523.2003.00965.x]
Vine-1 retrotransposon-based S-SAP (sequence-specific amplified polymorphism) for Vitis vinifera L genotyping
IMAZIO, Serena Anna;
2004
Abstract
The sequence-specific amplification polymorphism (S-SAP) method,derived from the amplified fragment length polymorphism (AFLP)technique, produces amplified fragments containing retrotransposonlong terminal repeat (LTR) sequence at one end and a host restrictionsite at the other. The development and application of this procedure tothe LTR of the Vine-1 element from grapevine is reported. Twoprimers derived from one of the LTR sequences flanking theretrotransposon were used in combination with MseI degeneratedprimers on 15 grapevine accessions. S-SAP results were compared withAFLP data. The heterozygosity and gene diversity values were higherfor S-SAP than for the AFLP procedure. Results show that S-SAPamplification is effective in identifying polymorphisms and defininggenetic distances among cultivars, and could be used for fingerprintingand for Traminer clone identification. To the contrary Vine-1retrotransposon-based S-SAP was not able to distinguish Pinotclones.
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