Synapsins are synaptic-vesicle-associated phosphoproteins implicated in the regulation of neurotransmitter release and excitability of neuronal networks. Mutation of synapsin genes in mouse and human causes epilepsy. To understand the role of the highly conserved synapsin domain E in the dynamics of release from mammalian inhibitory neurons, we generated mice that selectively overexpress the most conserved part of this domain in cerebellar Purkinje cells. At Purkinje-cell-nuclear-neuron synapses, transgenic mice were more resistant to depression induced by short or prolonged high-frequency stimulations. The increased synaptic performance was accompanied by accelerated release kinetics and shorter synaptic delay. Despite a marked decrease in the total number of synaptic vesicles, vesicles at the active zone were preserved or slightly increased. The data indicate that synapsin domain E increases synaptic efficiency by accelerating both the kinetics of exocytosis and the rate of synaptic vesicle cycling and decreasing depression at the inhibitory Purkinje-cell-nuclear-neuron synapse. These effects may increase the sensitivity of postsynaptic neurons to inhibition and thereby contribute to the inhibitory control of network activity

The synapsin domain E accelerates the exo-endocytotic cycle of synaptic vesicles in cerebellar Purkinje cells / A., Fassio; D., Merlo; Mapelli, Jonathan; A., Menegon; A., Corradi; M., Mete; S., Zappettini; G., Bonanno; F., Valtorta; E., D’Angelo; F., Benfenati. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - STAMPA. - 119:20(2006), pp. 4257-4268. [10.1242/jcs.03194]

The synapsin domain E accelerates the exo-endocytotic cycle of synaptic vesicles in cerebellar Purkinje cells

MAPELLI, Jonathan;
2006

Abstract

Synapsins are synaptic-vesicle-associated phosphoproteins implicated in the regulation of neurotransmitter release and excitability of neuronal networks. Mutation of synapsin genes in mouse and human causes epilepsy. To understand the role of the highly conserved synapsin domain E in the dynamics of release from mammalian inhibitory neurons, we generated mice that selectively overexpress the most conserved part of this domain in cerebellar Purkinje cells. At Purkinje-cell-nuclear-neuron synapses, transgenic mice were more resistant to depression induced by short or prolonged high-frequency stimulations. The increased synaptic performance was accompanied by accelerated release kinetics and shorter synaptic delay. Despite a marked decrease in the total number of synaptic vesicles, vesicles at the active zone were preserved or slightly increased. The data indicate that synapsin domain E increases synaptic efficiency by accelerating both the kinetics of exocytosis and the rate of synaptic vesicle cycling and decreasing depression at the inhibitory Purkinje-cell-nuclear-neuron synapse. These effects may increase the sensitivity of postsynaptic neurons to inhibition and thereby contribute to the inhibitory control of network activity
2006
119
20
4257
4268
The synapsin domain E accelerates the exo-endocytotic cycle of synaptic vesicles in cerebellar Purkinje cells / A., Fassio; D., Merlo; Mapelli, Jonathan; A., Menegon; A., Corradi; M., Mete; S., Zappettini; G., Bonanno; F., Valtorta; E., D’Angelo; F., Benfenati. - In: JOURNAL OF CELL SCIENCE. - ISSN 0021-9533. - STAMPA. - 119:20(2006), pp. 4257-4268. [10.1242/jcs.03194]
A., Fassio; D., Merlo; Mapelli, Jonathan; A., Menegon; A., Corradi; M., Mete; S., Zappettini; G., Bonanno; F., Valtorta; E., D’Angelo; F., Benfenati...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/687649
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