• We previously identified the lpa1 (low phytic acid) 280-10 line that carries amutation conferring a 90% reduction in phytic acid (InsP6) content. In contrast toother lpa mutants, lpa1(280-10) does not display negative pleiotropic effects. Inthe present paper, we have identified the mutated gene and analysed its impact onthe phytic acid pathway.• Here, we mapped the lpa1(280-10) mutation by bulk analysis on a segregatingF2 population, an then, by comparison with the soybean genome, we identifiedand sequenced a candidate gene. The InsP6 pathway was analysed by geneexpression and quantification of metabolites.• The mutated Pvmrp1(280-10) cosegregates with the lpa1(280-10) mutation,and the expression level of several genes of the InsP6 pathway are reduced in thelpa1(280-10) mutant as well as the inositol and raffinosaccharide content.PvMrp2, a very similar paralogue of PvMrp1 was also mapped and sequenced.• The lpa1 mutation in beans is likely the result of a defective Mrp1 gene (orthologousto the lpa genes AtMRP5 and ZmMRP4), while its Mrp2 paralog is not ableto complement the mutant phenotype in the seed. This mutation appears todown-regulate the InsP6 pathway at the transcriptional level, as well as alteringinositol-related metabolism and affecting ABA sensitivity.
A defective ABC transporter of the MRP family, responsible for the bean lpa1 mutation, affects the regulation of the phytic acid pathway, reduces seed myo-inositol and alters ABA sensitivity / D., Panzeri; E., Cassani; E., Doria; G., Tagliabue; Forti, Luca; B., Campion; R., Bollini; C. A., Brearley; R., Pilu; E., Nielsen; F., Sparvoli. - In: NEW PHYTOLOGIST. - ISSN 0028-646X. - STAMPA. - 191:1(2011), pp. 70-83. [10.1111/j.1469-8137.2011.03666.x]
A defective ABC transporter of the MRP family, responsible for the bean lpa1 mutation, affects the regulation of the phytic acid pathway, reduces seed myo-inositol and alters ABA sensitivity
FORTI, Luca;
2011
Abstract
• We previously identified the lpa1 (low phytic acid) 280-10 line that carries amutation conferring a 90% reduction in phytic acid (InsP6) content. In contrast toother lpa mutants, lpa1(280-10) does not display negative pleiotropic effects. Inthe present paper, we have identified the mutated gene and analysed its impact onthe phytic acid pathway.• Here, we mapped the lpa1(280-10) mutation by bulk analysis on a segregatingF2 population, an then, by comparison with the soybean genome, we identifiedand sequenced a candidate gene. The InsP6 pathway was analysed by geneexpression and quantification of metabolites.• The mutated Pvmrp1(280-10) cosegregates with the lpa1(280-10) mutation,and the expression level of several genes of the InsP6 pathway are reduced in thelpa1(280-10) mutant as well as the inositol and raffinosaccharide content.PvMrp2, a very similar paralogue of PvMrp1 was also mapped and sequenced.• The lpa1 mutation in beans is likely the result of a defective Mrp1 gene (orthologousto the lpa genes AtMRP5 and ZmMRP4), while its Mrp2 paralog is not ableto complement the mutant phenotype in the seed. This mutation appears todown-regulate the InsP6 pathway at the transcriptional level, as well as alteringinositol-related metabolism and affecting ABA sensitivity.File | Dimensione | Formato | |
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