We show that the activity of an ion channel is correlated with the phase state of the lipid bilayer hosting the channel By measuring unitary conductance dwell times and open probability of the K(+) channel KcsA as a function of temperature in lipid bilayers composed of POPE and POPG in different relative proportions we obtain that all those properties show a trend inversion when the bilayer is in the transition region between the liquid disordered and the solid ordered phase These data suggest that the physical properties of the lipid Mayer influence ion channel activity likely via a fine tuning of its conformations In a more general interpretative framework we suggest that other parameters such as pH ionic strength, and the action of amphiphilic drugs can affect the physical behavior of the lipid bilayer in a fashion similar to temperature changes resulting in functional changes of transmembrane proteins

Changes in Single K(+) Channel Behavior Induced by a Lipid Phase Transition / HM, Segger; L., Aldrovandi; ALESSANDRINI, Andrea; P., Facci. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - STAMPA. - 99:(2010), pp. 3675-3683. [10.1016/j.bpj.2010.10.042]

Changes in Single K(+) Channel Behavior Induced by a Lipid Phase Transition

ALESSANDRINI, Andrea;
2010

Abstract

We show that the activity of an ion channel is correlated with the phase state of the lipid bilayer hosting the channel By measuring unitary conductance dwell times and open probability of the K(+) channel KcsA as a function of temperature in lipid bilayers composed of POPE and POPG in different relative proportions we obtain that all those properties show a trend inversion when the bilayer is in the transition region between the liquid disordered and the solid ordered phase These data suggest that the physical properties of the lipid Mayer influence ion channel activity likely via a fine tuning of its conformations In a more general interpretative framework we suggest that other parameters such as pH ionic strength, and the action of amphiphilic drugs can affect the physical behavior of the lipid bilayer in a fashion similar to temperature changes resulting in functional changes of transmembrane proteins
99
3675
3683
Changes in Single K(+) Channel Behavior Induced by a Lipid Phase Transition / HM, Segger; L., Aldrovandi; ALESSANDRINI, Andrea; P., Facci. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - STAMPA. - 99:(2010), pp. 3675-3683. [10.1016/j.bpj.2010.10.042]
Hm, Segger; L., Aldrovandi; Alessandrini, Andrea; P., Facci
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/668047
Citazioni
  • ???jsp.display-item.citation.pmc??? 16
  • Scopus 35
  • ???jsp.display-item.citation.isi??? 34
social impact