The current view of the biological membrane is that in which lipids and proteins mutually interact to accomplish membrane functions. The lateral heterogeneity of the lipid bilayer can induce partitioning of membrane-associated proteins, favoring protein-protein interaction and influence signaling and trafficking. The Atomic Force Microscope allows to study the localization of membrane-associated proteins with respect to the lipid organization at the single molecule level and without the need for fluorescence staining. These features make AFM a technique of choice to study lipid/protein interactions in model systems or native membranes. Here we will review the technical aspects inherent to and the main results obtained by AFM in the study of protein partitioning in lipid domains concentrating in particular on GPI-anchored proteins, lipidated proteins, and transmembrane proteins. Whenever possible, we will also discuss the functional consequences of what has been imaged by Atomic Force Microscopy.

Unraveling lipid/protein interaction in model lipid bilayers by Atomic Force Microscopy / Alessandrini, Andrea. - In: JOURNAL OF MOLECULAR RECOGNITION. - ISSN 0952-3499. - STAMPA. - 24:3(2011), pp. 387-396. [10.1002/jmr.1083]

Unraveling lipid/protein interaction in model lipid bilayers by Atomic Force Microscopy

ALESSANDRINI, Andrea
2011

Abstract

The current view of the biological membrane is that in which lipids and proteins mutually interact to accomplish membrane functions. The lateral heterogeneity of the lipid bilayer can induce partitioning of membrane-associated proteins, favoring protein-protein interaction and influence signaling and trafficking. The Atomic Force Microscope allows to study the localization of membrane-associated proteins with respect to the lipid organization at the single molecule level and without the need for fluorescence staining. These features make AFM a technique of choice to study lipid/protein interactions in model systems or native membranes. Here we will review the technical aspects inherent to and the main results obtained by AFM in the study of protein partitioning in lipid domains concentrating in particular on GPI-anchored proteins, lipidated proteins, and transmembrane proteins. Whenever possible, we will also discuss the functional consequences of what has been imaged by Atomic Force Microscopy.
2011
24
3
387
396
Unraveling lipid/protein interaction in model lipid bilayers by Atomic Force Microscopy / Alessandrini, Andrea. - In: JOURNAL OF MOLECULAR RECOGNITION. - ISSN 0952-3499. - STAMPA. - 24:3(2011), pp. 387-396. [10.1002/jmr.1083]
Alessandrini, Andrea
File in questo prodotto:
File Dimensione Formato  
JMR2011.pdf

Accesso riservato

Tipologia: Versione pubblicata dall'editore
Dimensione 4.66 MB
Formato Adobe PDF
4.66 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/668045
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 40
  • ???jsp.display-item.citation.isi??? 39
social impact