The qualitative and quantitative characterization of propolis biomolecules is of interest. Recent analytical techniques can be applied to separate and quantify polyphenols in extracts used in medicine and nutraceuticals. It is also important to determine the typical ‘fingerprints’ of propolis for the reliable identification of a large number of polyphenolic components. Our laboratory has developed a multianalytical approach for the identification and chemical characterization of various extracts of propolis coming from different countries of origin. Accordingly, we have developed and validated spectrophotometric procedures for the quantification of bioactive substances in different types of propolis; such as HPLC-UV-ESI-MS and 1H- and 13C-NMR for an accurate assessment of the contents of these compounds in extract samples. The results show that the HPLC-UV-ESI-MS and NMR techniques are useful tools for the chemical classification of propolis and may provide alternatives to classical analytical phytochemistry to screen commercial preparations of propolis and to evaluate specific nutraceutical benefits.

New analytical approaches for the evaluation of the quality of propolis / Volpi, Nicola. - In: NUTRAFOODS. - ISSN 1827-8590. - STAMPA. - 10:(2011), pp. 2-3-55-60.

New analytical approaches for the evaluation of the quality of propolis

VOLPI, Nicola
2011

Abstract

The qualitative and quantitative characterization of propolis biomolecules is of interest. Recent analytical techniques can be applied to separate and quantify polyphenols in extracts used in medicine and nutraceuticals. It is also important to determine the typical ‘fingerprints’ of propolis for the reliable identification of a large number of polyphenolic components. Our laboratory has developed a multianalytical approach for the identification and chemical characterization of various extracts of propolis coming from different countries of origin. Accordingly, we have developed and validated spectrophotometric procedures for the quantification of bioactive substances in different types of propolis; such as HPLC-UV-ESI-MS and 1H- and 13C-NMR for an accurate assessment of the contents of these compounds in extract samples. The results show that the HPLC-UV-ESI-MS and NMR techniques are useful tools for the chemical classification of propolis and may provide alternatives to classical analytical phytochemistry to screen commercial preparations of propolis and to evaluate specific nutraceutical benefits.
2011
10
2-3
55-60
New analytical approaches for the evaluation of the quality of propolis / Volpi, Nicola. - In: NUTRAFOODS. - ISSN 1827-8590. - STAMPA. - 10:(2011), pp. 2-3-55-60.
Volpi, Nicola
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/649328
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