Five penicillinase producing Neisseria gonorrhoeae (PPNG) were isolated from urethral specimens of men admitted to the "Santa Chiara" Hospital (Trento, Italy). All strains proved to be resistant to penicillin and ampicillin, and sensitive to cefuroxime, erythromycin, tetracycline, spectinomycin, nalidixic acid and ciprofloxacin. PPNG plasmid profiles showed that four of the isolates carried the 3.2 MDa "Africa" plasmid and one the 4.5 MDa "Asia" plasmid, the two well-known phenotypes reported in the USA and Europe as well as in Asian and African countries. Membrane matings were performed using N. gonorrhoeae carrying the 24.5 MDa conjugative plasmid as donors and E. coli K12 J 53 as recipient. The transfer of beta-lactamic antibiotic resistance was supported by the presence of 4.5 or 3.2 MDa plasmid bands and by beta-lactamase production in the transconjugants. Restriction analysis of Asian and African plasmids is reported.

Study of five penicillinase producing Neisseria gonorrhoeae isolated in Italy / Sabia, Carla; Bondi, Moreno; Messi, Patrizia; DE NIEDERHAUSERN, Simona; Manicardi, Giuliano. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - ELETTRONICO. - 28:3(2005), pp. 223-229.

Study of five penicillinase producing Neisseria gonorrhoeae isolated in Italy.

SABIA, Carla;BONDI, Moreno;MESSI, Patrizia;DE NIEDERHAUSERN, Simona;MANICARDI, Giuliano
2005

Abstract

Five penicillinase producing Neisseria gonorrhoeae (PPNG) were isolated from urethral specimens of men admitted to the "Santa Chiara" Hospital (Trento, Italy). All strains proved to be resistant to penicillin and ampicillin, and sensitive to cefuroxime, erythromycin, tetracycline, spectinomycin, nalidixic acid and ciprofloxacin. PPNG plasmid profiles showed that four of the isolates carried the 3.2 MDa "Africa" plasmid and one the 4.5 MDa "Asia" plasmid, the two well-known phenotypes reported in the USA and Europe as well as in Asian and African countries. Membrane matings were performed using N. gonorrhoeae carrying the 24.5 MDa conjugative plasmid as donors and E. coli K12 J 53 as recipient. The transfer of beta-lactamic antibiotic resistance was supported by the presence of 4.5 or 3.2 MDa plasmid bands and by beta-lactamase production in the transconjugants. Restriction analysis of Asian and African plasmids is reported.
2005
28
3
223
229
Study of five penicillinase producing Neisseria gonorrhoeae isolated in Italy / Sabia, Carla; Bondi, Moreno; Messi, Patrizia; DE NIEDERHAUSERN, Simona; Manicardi, Giuliano. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - ELETTRONICO. - 28:3(2005), pp. 223-229.
Sabia, Carla; Bondi, Moreno; Messi, Patrizia; DE NIEDERHAUSERN, Simona; Manicardi, Giuliano
File in questo prodotto:
File Dimensione Formato  
THE NEW MICROBIOLOGICA 2005.pdf

Open access

Tipologia: Versione pubblicata dall'editore
Dimensione 163.95 kB
Formato Adobe PDF
163.95 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/648967
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact