Bioreduction of α,β-unsatured ketones and aldehydes by non-conventional yeast (NCY) whole-cells

Goretti, M.; Ponzoni, C.; Caselli, E.; Marchigiani, E.; Cramarossa, Maria Rita; Turchetti, B.; Forti, Luca; Buzzini, P.

doi:10.1016/j.biortech.2010.12.

The bioreduction of alfa,beta-unsaturated ketones (ketoisophorone, 2-methyl- and 3-methyl-cyclopentenone)and aldehydes [(S)-(-)-perillaldehyde and alfa-methyl-cinnamaldehyde] by 23 ‘‘non-conventional’’ yeasts (NCYs) belonging to 21 species of the genera Candida, Cryptococcus, Debaryomyces, Hanseniaspora, Kazachstania, Kluyveromyces, Lindnera, Nakaseomyces, Vanderwaltozyma, and Wickerhamomyces was reported. The results highlight the potential of NCYs as whole-cell biocatalysts for selective biotransformation of electron-poor alkenes. A few NCYs exhibited extremely high (>90%) or even total ketoisophorone and 2-methyl-cyclopentenone bioconversion yields via asymmetric reduction of the conjugated C=C bond catalyzed by enoate reductases. Catalytic efficiency declined after switching from ketones to aldehydes. High chemoselectivity due to low competing carbonyl reductases was also sometimes observed.

Bioreduction of α,β-unsatured ketones and aldehydes by non-conventional yeast (NCY) whole-cells / M., Goretti; C., Ponzoni; E., Caselli; E., Marchigiani; Cramarossa, Maria Rita; B., Turchetti; Forti, Luca; P., Buzzini. - In: BIORESOURCE TECHNOLOGY. - ISSN 0960-8524. - STAMPA. - 102(2011), pp. 3993-3998.

Data di pubblicazione:	2011
Titolo:	Bioreduction of α,β-unsatured ketones and aldehydes by non-conventional yeast (NCY) whole-cells
Autore/i:	M., Goretti; C., Ponzoni; E., Caselli; E., Marchigiani; Cramarossa, Maria Rita; B., Turchetti; Forti, Luca; P., Buzzini
Autore/i UNIMORE:	M. Goretti C. Ponzoni E. Caselli E. Marchigiani CRAMAROSSA, Maria Rita B. Turchetti FORTI, Luca P. Buzzini mostra contributor esterni nascondi contributor esterni
Digital Object Identifier (DOI):	http://dx.doi.org/10.1016/j.biortech.2010.12.
Rivista:	BIORESOURCE TECHNOLOGY
Volume:	102
Pagina iniziale:	3993
Pagina finale:	3998
Codice identificativo ISI:	WOS:000287786300001
Codice identificativo Scopus:	2-s2.0-79151470478
Codice identificativo Pubmed:	21232941
Citazione:	Bioreduction of α,β-unsatured ketones and aldehydes by non-conventional yeast (NCY) whole-cells / M., Goretti; C., Ponzoni; E., Caselli; E., Marchigiani; Cramarossa, Maria Rita; B., Turchetti; Forti, Luca; P., Buzzini. - In: BIORESOURCE TECHNOLOGY. - ISSN 0960-8524. - STAMPA. - 102(2011), pp. 3993-3998.
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