The bioreduction of alfa,beta-unsaturated ketones (ketoisophorone, 2-methyl- and 3-methyl-cyclopentenone)and aldehydes [(S)-(-)-perillaldehyde and alfa-methyl-cinnamaldehyde] by 23 ‘‘non-conventional’’ yeasts (NCYs) belonging to 21 species of the genera Candida, Cryptococcus, Debaryomyces, Hanseniaspora, Kazachstania, Kluyveromyces, Lindnera, Nakaseomyces, Vanderwaltozyma, and Wickerhamomyces was reported. The results highlight the potential of NCYs as whole-cell biocatalysts for selective biotransformation of electron-poor alkenes. A few NCYs exhibited extremely high (>90%) or even total ketoisophorone and 2-methyl-cyclopentenone bioconversion yields via asymmetric reduction of the conjugated C=C bond catalyzed by enoate reductases. Catalytic efficiency declined after switching from ketones to aldehydes. High chemoselectivity due to low competing carbonyl reductases was also sometimes observed.
Bioreduction of α,β-unsatured ketones and aldehydes by non-conventional yeast (NCY) whole-cells / M., Goretti; C., Ponzoni; E., Caselli; E., Marchigiani; Cramarossa, Maria Rita; B., Turchetti; Forti, Luca; P., Buzzini. - In: BIORESOURCE TECHNOLOGY. - ISSN 0960-8524. - STAMPA. - 102:5(2011), pp. 3993-3998. [10.1016/j.biortech.2010.12.062]
Bioreduction of α,β-unsatured ketones and aldehydes by non-conventional yeast (NCY) whole-cells
CRAMAROSSA, Maria Rita;FORTI, Luca;
2011
Abstract
The bioreduction of alfa,beta-unsaturated ketones (ketoisophorone, 2-methyl- and 3-methyl-cyclopentenone)and aldehydes [(S)-(-)-perillaldehyde and alfa-methyl-cinnamaldehyde] by 23 ‘‘non-conventional’’ yeasts (NCYs) belonging to 21 species of the genera Candida, Cryptococcus, Debaryomyces, Hanseniaspora, Kazachstania, Kluyveromyces, Lindnera, Nakaseomyces, Vanderwaltozyma, and Wickerhamomyces was reported. The results highlight the potential of NCYs as whole-cell biocatalysts for selective biotransformation of electron-poor alkenes. A few NCYs exhibited extremely high (>90%) or even total ketoisophorone and 2-methyl-cyclopentenone bioconversion yields via asymmetric reduction of the conjugated C=C bond catalyzed by enoate reductases. Catalytic efficiency declined after switching from ketones to aldehydes. High chemoselectivity due to low competing carbonyl reductases was also sometimes observed.
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