Short intense electrical pulses transiently increase the permeability of the cell membrane, an effect known as electroporation. This can be combined with antiblastic drugs for ablation of tumours of the skin and subcutaneous tissue. The aim of this study was to test the efficacy of electroporation when applied to bone and to understand whether the presence of mineralised trabeculae would affect the capability of the electric field to porate the membrane of bone cells. Different levels of electrical field were applied to the femoral bone of rabbits. The field distribution and modelling were simulated by computer. Specimens of bone from treated and control rabbits were obtained for histology, histomorphometry and biomechanical testing. After seven days, the area of ablation had increased in line with the number of pulses and/or with the amplitude of the electrical field applied. The osteogenic activity in the ablated area had recovered by 30 days. Biomechanical testing showed structural integrity of the bone at both times. Electroporation using the appropriate combination of voltage and pulses induced ablation of bone cells without affecting the recovery of osteogenic activity. It can be an effective treatment in bone and when used in combination with drugs, an option for the treatment of metastases.

Ablation of bone cells by electroporation / Fini, M; Tschon, M; Ronchetti, M; Cavani, Francesco; Bianchi, G; Mercuri, M; Alberghini, M; Cadossi, R.. - In: JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME. - ISSN 0301-620X. - STAMPA. - 92(11):(2010), pp. 1614-1620. [10.1302/0301-620X.92B11.24664]

Ablation of bone cells by electroporation

CAVANI, Francesco;
2010

Abstract

Short intense electrical pulses transiently increase the permeability of the cell membrane, an effect known as electroporation. This can be combined with antiblastic drugs for ablation of tumours of the skin and subcutaneous tissue. The aim of this study was to test the efficacy of electroporation when applied to bone and to understand whether the presence of mineralised trabeculae would affect the capability of the electric field to porate the membrane of bone cells. Different levels of electrical field were applied to the femoral bone of rabbits. The field distribution and modelling were simulated by computer. Specimens of bone from treated and control rabbits were obtained for histology, histomorphometry and biomechanical testing. After seven days, the area of ablation had increased in line with the number of pulses and/or with the amplitude of the electrical field applied. The osteogenic activity in the ablated area had recovered by 30 days. Biomechanical testing showed structural integrity of the bone at both times. Electroporation using the appropriate combination of voltage and pulses induced ablation of bone cells without affecting the recovery of osteogenic activity. It can be an effective treatment in bone and when used in combination with drugs, an option for the treatment of metastases.
92(11)
1614
1620
Ablation of bone cells by electroporation / Fini, M; Tschon, M; Ronchetti, M; Cavani, Francesco; Bianchi, G; Mercuri, M; Alberghini, M; Cadossi, R.. - In: JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME. - ISSN 0301-620X. - STAMPA. - 92(11):(2010), pp. 1614-1620. [10.1302/0301-620X.92B11.24664]
Fini, M; Tschon, M; Ronchetti, M; Cavani, Francesco; Bianchi, G; Mercuri, M; Alberghini, M; Cadossi, R.
File in questo prodotto:
File Dimensione Formato  
Electroporation.pdf

non disponibili

Tipologia: Post-print dell'autore (bozza post referaggio)
Dimensione 2.78 MB
Formato Adobe PDF
2.78 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/646548
Citazioni
  • ???jsp.display-item.citation.pmc??? 19
  • Scopus 42
  • ???jsp.display-item.citation.isi??? 38
social impact