A collection of 28 Osteospermum clones and cultivated varieties ofdi}erent origin were evaluated by random amplifed polymorphic DNA (RAPD) analysis. All the clones were identifed by 12 decamers selectedfrom a set of 29. This is the first characterization by molecular markersof the genetic material of Osteospermum. The level of genetic diversityamong genotypes was assayed and all the accessions tested were thenclassified into six groups by UPGMA cluster analysis. The clustering ofgenotypes using the RAPD data proved to be in accordance with theirbreeding group origin. RAPD analysis can therefore be a useful toolfor evaluating genetic variability in other Osteospermum germplasmcollections for breeding purposes and for protecting intellectual property rights of improved varieties
Genetic diversity in Osteospermum as revealed by random amplified polymorphic DNA analysis / Faccioli, P.; V., Terzi; Pecchioni, Nicola; T., Berio; A., Giovannini; A., Allavena. - In: PLANT BREEDING. - ISSN 0179-9541. - STAMPA. - 119:4(2000), pp. 351-355. [10.1046/j.1439-0523.2000.00511.x]
Genetic diversity in Osteospermum as revealed by random amplified polymorphic DNA analysis
PECCHIONI, Nicola;
2000
Abstract
A collection of 28 Osteospermum clones and cultivated varieties ofdi}erent origin were evaluated by random amplifed polymorphic DNA (RAPD) analysis. All the clones were identifed by 12 decamers selectedfrom a set of 29. This is the first characterization by molecular markersof the genetic material of Osteospermum. The level of genetic diversityamong genotypes was assayed and all the accessions tested were thenclassified into six groups by UPGMA cluster analysis. The clustering ofgenotypes using the RAPD data proved to be in accordance with theirbreeding group origin. RAPD analysis can therefore be a useful toolfor evaluating genetic variability in other Osteospermum germplasmcollections for breeding purposes and for protecting intellectual property rights of improved varieties
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