EnglishInsects protect themselves from microbial invaders by two main immune activities, namely the cellular and humoral reactions. Phenoloxidases are oxidative enzymes that have an important role in both cell-mediated and humoral immunity. In this study, the purification and biochemical characterization of a phenoloxidase from the hemocytes of the Sunn pest Eurygaster integriceps Puton (Hemiptera: Scutelleridae) was carried out. After the final purification step, the enzyme was purified 7.31-fold with a recovery of 3.94% and a specific activity of 4.95 U/mg protein. Results of the biochemical characterization showed that the purified phenoloxidase has a maximum activity at pH 6 and at 30-35 ˚C and is stable for 24-36 hours. Divalent cations such as Ca2+ and Cu2+ significantly increased the enzymatic activity and synthetic inhibitors such as phenylthiourea significantly decreased it. The purified phenoloxidase has a molecular weight of 22 kDa. The current paper represents a further step towards the characterization of humoral immunity of E. integriceps in order to develop new strategies for the biological control of the Sunn pest
Purification and characterization of phenoloxidase from the hemocytes of Eurygaster integriceps Puton (Hemiptera: Scutelleridae) / A., Zibaee; A. R., Bandani; Malagoli, Davide. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART B, BIOCHEMISTRY & MOLECULAR BIOLOGY. - ISSN 1096-4959. - STAMPA. - 158(2011), pp. 117-123. [10.1016/j.cbpb.2010.10.006]
| Data di pubblicazione: | 2011 | |
| Titolo: | Purification and characterization of phenoloxidase from the hemocytes of Eurygaster integriceps Puton (Hemiptera: Scutelleridae) | |
| Autore/i: | A., Zibaee; A. R., Bandani; Malagoli, Davide | |
| Autore/i UNIMORE: | ||
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.cbpb.2010.10.006 | |
| Rivista: | ||
| Volume: | 158 | |
| Pagina iniziale: | 117 | |
| Pagina finale: | 123 | |
| Codice identificativo ISI: | WOS:000286027000014 | |
| Codice identificativo Scopus: | 2-s2.0-78649636018 | |
| Codice identificativo Pubmed: | 20970518 | |
| Citazione: | Purification and characterization of phenoloxidase from the hemocytes of Eurygaster integriceps Puton (Hemiptera: Scutelleridae) / A., Zibaee; A. R., Bandani; Malagoli, Davide. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART B, BIOCHEMISTRY & MOLECULAR BIOLOGY. - ISSN 1096-4959. - STAMPA. - 158(2011), pp. 117-123. [10.1016/j.cbpb.2010.10.006] | |
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