Impression materials are largely used to record the geometry of dental tissue. Hence, the assessment of their possible cytotoxicity is a necessary step in the evaluation of their biocompatibility. The present study is carried out to evaluate the cytotoxicity of a new elastomeric sterile and radiopaque impression material. Human gingival fibroblasts, cultured in vitro are exposed directly to Elite Implant in three different viscosities, heavy, medium, and light. At 3, 9, 24, 48, and 72 h, the cellular proliferation is evaluated. In parallel, human gingival fibroblasts are exposed indirectly by means of fluid extracts of Elite Implant. The cellular viability is evaluated by 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide, (MTT) assay (Sigma, St Louis, Mo). The gingival fibroblasts proliferation and viability are unaffected by the presence of Elite Implant. This new impression material may represent a safe medical device for clinical and surgical applications. In addition, this material is radiopaque and, thus, can be identified radiographically.

A new generation of sterile and radiopaque impression materials - an in vitro cytotoxicity study / Coppi, C.; Paolinelli Devincenzi, C.; Bortolini, Sergio; Consolo, Ugo; Tiozzo, Roberta. - In: JOURNAL OF BIOMATERIALS APPLICATIONS. - ISSN 0885-3282. - ELETTRONICO. - Volume: 22 Issue: 1(2007), pp. 83-95.

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