Echinacea purpurea (L.) Moench extracts are used in the production of standardized herbal medicines for the prevention and treatment of upper respiratory infections. Unsaturated N-alkylamide lipids, the main constituents of E. purpurea and E. angustifolia preparations capable of activating the cannabinoid receptor type-2 (CB2) have been suggested to play a role as potential anti-inflammatory and immune-modulatory principles. Here we show that ethanolic E. purpurea radix and herba extracts produce synergistic pharmacological effects on the endocannabinoid system in vitro. Superadditive action of N-alkylamide combinations were seen at the level of intracellular calcium release as a function of CB2 receptor activation. Likewise, synergism of the radix and herba tinctures was observed in experiments measuring LPS-stimulated cytokine expression from human PBMCs. While the expression of the anti-inflammatory cytokine IL-10 was significantly superstimulated, the expression of the pro-inflammatory TNF-alpha protein was inhibited more strongly upon combination of the extracts. We show that N-alkylamides act in concert and exert pleiotropic effects modulating the endocannabinoid system by simultaneously targeting the CB2 receptor, endocannabinoid transport and degradation.

Synergistic immunopharmacological effects of N-alkylamides in Echinacea purpurea herbal extracts / A., Chicca; S., Raduner; Pellati, Federica; T., Strompen; K. H., Altmann; R., Schoop; J., Gertsch. - In: PLANTA MEDICA. - ISSN 0032-0943. - STAMPA. - 75:(2009), pp. 991-991. ((Intervento presentato al convegno 57th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research tenutosi a Ginevra, Svizzera nel August 16-20 2009.

Synergistic immunopharmacological effects of N-alkylamides in Echinacea purpurea herbal extracts

PELLATI, Federica;
2009

Abstract

Echinacea purpurea (L.) Moench extracts are used in the production of standardized herbal medicines for the prevention and treatment of upper respiratory infections. Unsaturated N-alkylamide lipids, the main constituents of E. purpurea and E. angustifolia preparations capable of activating the cannabinoid receptor type-2 (CB2) have been suggested to play a role as potential anti-inflammatory and immune-modulatory principles. Here we show that ethanolic E. purpurea radix and herba extracts produce synergistic pharmacological effects on the endocannabinoid system in vitro. Superadditive action of N-alkylamide combinations were seen at the level of intracellular calcium release as a function of CB2 receptor activation. Likewise, synergism of the radix and herba tinctures was observed in experiments measuring LPS-stimulated cytokine expression from human PBMCs. While the expression of the anti-inflammatory cytokine IL-10 was significantly superstimulated, the expression of the pro-inflammatory TNF-alpha protein was inhibited more strongly upon combination of the extracts. We show that N-alkylamides act in concert and exert pleiotropic effects modulating the endocannabinoid system by simultaneously targeting the CB2 receptor, endocannabinoid transport and degradation.
75
991
991
A., Chicca; S., Raduner; Pellati, Federica; T., Strompen; K. H., Altmann; R., Schoop; J., Gertsch
Synergistic immunopharmacological effects of N-alkylamides in Echinacea purpurea herbal extracts / A., Chicca; S., Raduner; Pellati, Federica; T., Strompen; K. H., Altmann; R., Schoop; J., Gertsch. - In: PLANTA MEDICA. - ISSN 0032-0943. - STAMPA. - 75:(2009), pp. 991-991. ((Intervento presentato al convegno 57th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research tenutosi a Ginevra, Svizzera nel August 16-20 2009.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/640528
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