The retina is subject to degenerative conditions leading to blindness. Although retinal regeneration is possible in lower vertebrates, it does not occur in the adult mammalian retina. Retinal stem cell (RSC) research offers unique opportunities for developing clinical application for therapy. The ciliary body of adult mammals represents a source of quiescent RSC. These neural progenitors have a limited self-renewal potential in vitro but this can be improved by mitogens. Pigment Epithelium Derived Factor (PEDF), a member of the serpin gene family, is synthesized and secreted by retinal pigment epithelium (RPE) cells. We tested combinations of PEDF with fibroblast growth factor (FGF) during RSC growth to evaluate self-renewal and subsequent differentiation into retinal-like neuronal cell types. Medium supplemented with FGF + PEDF enhanced the RSC yield and more interestingly allowed expansion of the culture by increasing secondary retinal neurospheres after the 1st passage. This effect was accompanied by cell proliferation as revealed by BrdU incorporation. PEDF usage did not affect rod-like differentiation potential. This was demonstrated by immunofluorescence analysis of Rhodopsin and Pde6b that were found similarly expressed in cells derived from FGF or FGF + PEDF cultured RSC. Our studies suggest a possible application of PEDF in Retinal Stem Cell culture and transplantation.

PEDF Promotes Retinal Neurosphere Formation and Expansion In Vitro / DE MARZO, Anna; Aruta, CLAUDIA GAETANA; Marigo, Valeria. - STAMPA. - 664:(2010), pp. 621-630. ((Intervento presentato al convegno 13th International Symposium on Retinal Degeneration tenutosi a Emeishan, PEOPLES R CHINA nel SEP 18-23, 2008 [10.1007/978-1-4419-1399-9_71].

PEDF Promotes Retinal Neurosphere Formation and Expansion In Vitro

DE MARZO, Anna;ARUTA, CLAUDIA GAETANA;MARIGO, Valeria
2010

Abstract

The retina is subject to degenerative conditions leading to blindness. Although retinal regeneration is possible in lower vertebrates, it does not occur in the adult mammalian retina. Retinal stem cell (RSC) research offers unique opportunities for developing clinical application for therapy. The ciliary body of adult mammals represents a source of quiescent RSC. These neural progenitors have a limited self-renewal potential in vitro but this can be improved by mitogens. Pigment Epithelium Derived Factor (PEDF), a member of the serpin gene family, is synthesized and secreted by retinal pigment epithelium (RPE) cells. We tested combinations of PEDF with fibroblast growth factor (FGF) during RSC growth to evaluate self-renewal and subsequent differentiation into retinal-like neuronal cell types. Medium supplemented with FGF + PEDF enhanced the RSC yield and more interestingly allowed expansion of the culture by increasing secondary retinal neurospheres after the 1st passage. This effect was accompanied by cell proliferation as revealed by BrdU incorporation. PEDF usage did not affect rod-like differentiation potential. This was demonstrated by immunofluorescence analysis of Rhodopsin and Pde6b that were found similarly expressed in cells derived from FGF or FGF + PEDF cultured RSC. Our studies suggest a possible application of PEDF in Retinal Stem Cell culture and transplantation.
RETINAL DEGENERATIVE DISEASES: LABORATORY AND THERAPEUTIC INVESTIGATIONS
978-1-4419-1398-2
SPRINGER-VERLAG BERLIN
STATI UNITI D'AMERICA
PEDF Promotes Retinal Neurosphere Formation and Expansion In Vitro / DE MARZO, Anna; Aruta, CLAUDIA GAETANA; Marigo, Valeria. - STAMPA. - 664:(2010), pp. 621-630. ((Intervento presentato al convegno 13th International Symposium on Retinal Degeneration tenutosi a Emeishan, PEOPLES R CHINA nel SEP 18-23, 2008 [10.1007/978-1-4419-1399-9_71].
DE MARZO, Anna; Aruta, CLAUDIA GAETANA; Marigo, Valeria
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/638782
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