Background: Microparticles are used for controlleddrug delivery. With the aim of improving both bioavailabilityand tamoxifen selective toxicity, the activity of tamoxifenembedded in calcium alginate/chitosan microparticles wasstudied. Materials and Methods: Tamoxifen alone andembedded in microparticles prepared with sodium alginatefrom Kelco (62% mannuronic acid and 38% guluronic acid)and from Fluka (30% mannuronic acid and 70% guluronicacid) was added to MCF-7 and Vero cultures and evaluated forantiproliferative activity by the MTT test. Results: The use ofKelco or Fluka alginate resulted in different LD50 values onVero and MCF-7 cultures, showing a higher cytotoxicitytoward Vero cells treated with tamoxifen embedded in Kelcomicroparticles (25 μM vs. 48 μM on MCF-7 cells) but aselective toxicity with Fluka microparticles (25 μM and 10 μMon Vero and MCF-7 cells respectively). Conclusion:Microparticle formulation may improve selective toxicityaccording to the alginate employed: differences in the chemicalalginate composition can dramatically change both drugactivity and toxicity.

Role of the Pharmaceutical Excipients in the Tamoxifen Activity on MCF-7 and Vero Cell Cultures / Rossi, Tiziana; Iannuccelli, Valentina; Coppi, Gilberto; E., Bruni; Baggio, Giosuè Gabriele. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - STAMPA. - 29:11(2009), pp. 4529-4534.

Role of the Pharmaceutical Excipients in the Tamoxifen Activity on MCF-7 and Vero Cell Cultures

ROSSI, Tiziana;IANNUCCELLI, Valentina;COPPI, Gilberto;BAGGIO, Giosuè Gabriele
2009

Abstract

Background: Microparticles are used for controlleddrug delivery. With the aim of improving both bioavailabilityand tamoxifen selective toxicity, the activity of tamoxifenembedded in calcium alginate/chitosan microparticles wasstudied. Materials and Methods: Tamoxifen alone andembedded in microparticles prepared with sodium alginatefrom Kelco (62% mannuronic acid and 38% guluronic acid)and from Fluka (30% mannuronic acid and 70% guluronicacid) was added to MCF-7 and Vero cultures and evaluated forantiproliferative activity by the MTT test. Results: The use ofKelco or Fluka alginate resulted in different LD50 values onVero and MCF-7 cultures, showing a higher cytotoxicitytoward Vero cells treated with tamoxifen embedded in Kelcomicroparticles (25 μM vs. 48 μM on MCF-7 cells) but aselective toxicity with Fluka microparticles (25 μM and 10 μMon Vero and MCF-7 cells respectively). Conclusion:Microparticle formulation may improve selective toxicityaccording to the alginate employed: differences in the chemicalalginate composition can dramatically change both drugactivity and toxicity.
2009
29
11
4529
4534
Role of the Pharmaceutical Excipients in the Tamoxifen Activity on MCF-7 and Vero Cell Cultures / Rossi, Tiziana; Iannuccelli, Valentina; Coppi, Gilberto; E., Bruni; Baggio, Giosuè Gabriele. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - STAMPA. - 29:11(2009), pp. 4529-4534.
Rossi, Tiziana; Iannuccelli, Valentina; Coppi, Gilberto; E., Bruni; Baggio, Giosuè Gabriele
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/638579
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