Study of the bacterial community affiliated to Hyalesthes obsoletus, the insect vector of “bois noir” phytoplasma of grape

Gonella, E.; Negri, I.; Marzorati, M.; Brusetti, L.; Pajoro, M.; Mandrioli, Mauro; Tedeschi, R.; Daffonchio, D.; Alma, A.

Grape yellows caused by phytoplasmas afflict several important wine-producing areas of Europe. A grape yellows with increasingincidence in European vineyards is “bois noir” (BN), caused by ‘Candidatus Phytoplasma solani’. Its vector is the planthopperHyalesthes obsoletus Signoret (Hemiptera Cixiidae), occasionally feeding on grapevine. An innovative strategy for reducing thediffusion of the disease could be symbiotic control, exploiting the action of symbiotic microorganisms of the insect host. To investigatethe occurrence of possible microbial candidates for symbiotic control we performed a molecular characterization of thebacteria associated to H. obsoletus. Length heterogeneity PCR was applied for a preliminary population screening. Taxonomicaffiliations of the bacterial species were analyzed by denaturing gradient gel electrophoresis, showing, within the microbial diversity,the intracellular reproductive parasite Wolbachia pipientis and a Bacteroidetes symbiont with 92% nt identity with ‘CandidatusSulcia muelleri’. PCR essays specific for these bacteria showed they co-localize in several organs of H. obsoletus. Fluorescentin situ hybridization was performed to assess the distribution of these microorganisms within the insect body, showing interestinglocalization patterns, particularly in insect gonads and salivary glands. These results could be a starting point for a deeper investigationof functions and relationships between microbial species.

Study of the bacterial community affiliated to Hyalesthes obsoletus, the insect vector of “bois noir” phytoplasma of grape / E., Gonella; I., Negri; M., Marzorati; L., Brusetti; M., Pajoro; Mandrioli, Mauro; R., Tedeschi; D., Daffonchio; A., Alma. - In: BULLETIN OF INSECTOLOGY. - ISSN 1721-8861. - STAMPA. - 61(2008), pp. 221-222.

Data di pubblicazione:	2008
Titolo:	Study of the bacterial community affiliated to Hyalesthes obsoletus, the insect vector of “bois noir” phytoplasma of grape
Autore/i:	E., Gonella; I., Negri; M., Marzorati; L., Brusetti; M., Pajoro; Mandrioli, Mauro; R., Tedeschi; D., Daffonchio; A., Alma
Autore/i UNIMORE:	E. Gonella I. Negri M. Marzorati L. Brusetti M. Pajoro MANDRIOLI, Mauro R. Tedeschi D. Daffonchio A. Alma mostra contributor esterni nascondi contributor esterni
Rivista:	BULLETIN OF INSECTOLOGY
Volume:	61
Pagina iniziale:	221
Pagina finale:	222
Codice identificativo ISI:	WOS:000257657000075
Codice identificativo Scopus:	2-s2.0-77952037173
Citazione:	Study of the bacterial community affiliated to Hyalesthes obsoletus, the insect vector of “bois noir” phytoplasma of grape / E., Gonella; I., Negri; M., Marzorati; L., Brusetti; M., Pajoro; Mandrioli, Mauro; R., Tedeschi; D., Daffonchio; A., Alma. - In: BULLETIN OF INSECTOLOGY. - ISSN 1721-8861. - STAMPA. - 61(2008), pp. 221-222.
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