An orange- coloured (ORP) protein from D.gigas, a sulphate reducer bacterium, has been previously shown by extended X-ray absorption fine structure to contain a novel mixed-metal sulphide cluster of the type of [S2MoS2CuS2MoS2]. We report here the purification and the biochemical/spectroscopic characterisation of this novel protein. The cluster is not covalently bound to the polypeptide chain. The gene sequence coding for ORP as well as the amino acid sequence was determined. The putative biologic function of ORP was discussed.

Antagonists Mo and Cu in a heterometallic cluster present on a novel protein (orange protein) isolated from Desulfovibrio gigas / S. A., Bursakov; Gavel, O. Y. u.; DI ROCCO, Giulia; J., Lampreia; J., Calvete; A. S., Pereira; J. J. G., Moura; I., Moura. - In: BIOCHEMISTRY. - ISSN 0006-2960. - ELETTRONICO. - 98:(2004), pp. 833-840. [10.1016/j.jinorgbio.2003.12.002]

Antagonists Mo and Cu in a heterometallic cluster present on a novel protein (orange protein) isolated from Desulfovibrio gigas

DI ROCCO, Giulia;
2004

Abstract

An orange- coloured (ORP) protein from D.gigas, a sulphate reducer bacterium, has been previously shown by extended X-ray absorption fine structure to contain a novel mixed-metal sulphide cluster of the type of [S2MoS2CuS2MoS2]. We report here the purification and the biochemical/spectroscopic characterisation of this novel protein. The cluster is not covalently bound to the polypeptide chain. The gene sequence coding for ORP as well as the amino acid sequence was determined. The putative biologic function of ORP was discussed.
2004
98
833
840
Antagonists Mo and Cu in a heterometallic cluster present on a novel protein (orange protein) isolated from Desulfovibrio gigas / S. A., Bursakov; Gavel, O. Y. u.; DI ROCCO, Giulia; J., Lampreia; J., Calvete; A. S., Pereira; J. J. G., Moura; I., Moura. - In: BIOCHEMISTRY. - ISSN 0006-2960. - ELETTRONICO. - 98:(2004), pp. 833-840. [10.1016/j.jinorgbio.2003.12.002]
S. A., Bursakov; Gavel, O. Y. u.; DI ROCCO, Giulia; J., Lampreia; J., Calvete; A. S., Pereira; J. J. G., Moura; I., Moura
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/625016
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