EnglishFluorophore-assisted carbohydrate electrophoresis (FACE) was applied to determine the molecular mass (M) values of various chondroitin sulfate (CS) samples. After labeling with 8-aminonaphthalene-1,3,6-trisulfonic acid (ANTS), FACE was able to resolve each CS sample as a discrete band depending on the M value. After densitometric acquisition, the migration distance of each CS standard was acquired and the third grade polynomial calibration standard curve was determined by plotting the logarithms of the M values as a function of migration ratio. Purified CS samples of different origin and the European Pharmacopeia CS standard were analyzed by both FACE and conventional high-performance size-exclusion liquid chromatography (HPSEC) methods. The molecular weight value on the top of the chromatographic peak (Mp), the number-average Mn, weight-average Mw, and polydispersity (Mw/Mn) were examined by both techniques and found to be quite similar. This study demonstrates that FACE analysis is a suitable, sensitive and simple method for the determination of the M values of CS macromolecules with possible utilization in virtually any kind of research and development such as quality control laboratories.
Determination of molecular mass values of chondroitin sulfates by fluorophore-assisted carbohydrate electrophoresis (FACE) / D., Buzzega; Maccari, Francesca; Volpi, Nicola. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 51(2010), pp. 969-972. [10.1016/j.jpba.2009.10.015]
| Data di pubblicazione: | 2010 | |
| Titolo: | Determination of molecular mass values of chondroitin sulfates by fluorophore-assisted carbohydrate electrophoresis (FACE) | |
| Autore/i: | D., Buzzega; Maccari, Francesca; Volpi, Nicola | |
| Autore/i UNIMORE: | ||
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.jpba.2009.10.015 | |
| Rivista: | ||
| Volume: | 51 | |
| Pagina iniziale: | 969 | |
| Pagina finale: | 972 | |
| Codice identificativo ISI: | WOS:000273510600025 | |
| Codice identificativo Scopus: | 2-s2.0-72149119707 | |
| Codice identificativo Pubmed: | 19931355 | |
| Citazione: | Determination of molecular mass values of chondroitin sulfates by fluorophore-assisted carbohydrate electrophoresis (FACE) / D., Buzzega; Maccari, Francesca; Volpi, Nicola. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 51(2010), pp. 969-972. [10.1016/j.jpba.2009.10.015] | |
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