The adsorption of native, wild-type and engineered cytochrome c on sodium-exchanged kaolinite was investigated by spectroscopic means. The variants of yeast cytochrome c were obtained replacing surface lysines in position 72, 73 and 79 with alanine residues. All proteins are strongly adsorbed onto kaolinite. In particular, the presence of the lysine residue in position 73 remarkably favors adsorption. A detailed characterization of the thermodynamic aspects of the adsorption process has been performed. Most notably, adsorbed cytochrome c maintains its moderate peroxidase activity against guaiacol. This investigation is prodromal to the exploitation of the catalytic activity of engineered cytochrome c immobilized on a polydisperse system.

Thermodynamic Aspects of the Adsorption of Cytochrome c and Its Mutants on Kaolinite / Castellini, Elena; Ranieri, Antonio; Domenico A., Simari; Di Rocco, Giulia. - In: LANGMUIR. - ISSN 0743-7463. - ELETTRONICO. - 25(2009), pp. 6849-6855. [10.1021/la9001016]

Thermodynamic Aspects of the Adsorption of Cytochrome c and Its Mutants on Kaolinite

CASTELLINI, Elena;RANIERI, Antonio;DI ROCCO, Giulia
2009

Abstract

The adsorption of native, wild-type and engineered cytochrome c on sodium-exchanged kaolinite was investigated by spectroscopic means. The variants of yeast cytochrome c were obtained replacing surface lysines in position 72, 73 and 79 with alanine residues. All proteins are strongly adsorbed onto kaolinite. In particular, the presence of the lysine residue in position 73 remarkably favors adsorption. A detailed characterization of the thermodynamic aspects of the adsorption process has been performed. Most notably, adsorbed cytochrome c maintains its moderate peroxidase activity against guaiacol. This investigation is prodromal to the exploitation of the catalytic activity of engineered cytochrome c immobilized on a polydisperse system.
25
6849
6855
Thermodynamic Aspects of the Adsorption of Cytochrome c and Its Mutants on Kaolinite / Castellini, Elena; Ranieri, Antonio; Domenico A., Simari; Di Rocco, Giulia. - In: LANGMUIR. - ISSN 0743-7463. - ELETTRONICO. - 25(2009), pp. 6849-6855. [10.1021/la9001016]
Castellini, Elena; Ranieri, Antonio; Domenico A., Simari; Di Rocco, Giulia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/620315
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