Yessotoxin (YTX) is a sulphated polyether compound produced by some species of dinoflagellate algae, that can be accumulated in bivalve mollusks and ingested by humans upon eating contaminated shellfish. Experiments in mice have demonstrated the lethal effect of YTX after intraperitoneal injection, whereas its oral administration has only limited acute toxicity, coupled with an alteration of plasma membrane protein turnover in the colon of the animals. In vitro studies have shown that this effect is due to the inhibition of endocytosis induced by the toxin. In this work, we investigated the effects of YTX on phagocytosis by using the J774 macrophage cell line. We found that macrophages exposed to 10 or 1nM YTX display a reduced phagocytic activity against Candida albicans; moreover, phagosome maturation is also inhibited in these cells. Such results were confirmed with resident peritoneal macrophages from normal mice. The inhibition of both phagocytosis and phagosome maturation likely involves cytoskeletal alterations, since a striking rearrangement of the F-actin organization occurs in YTX-treated J774 macrophages. Surprisingly, YTX also enhances cytokine production (TNF-alpha, MIP-1alpha and MIP-2) by J774 macrophages. Overall, our results show that low doses of YTX significantly affect both effector and secretory functions of macrophages.

Yessotoxin inhibits phagocytic activity of macrophages / Orsi, Carlotta Francesca; Colombari, Bruna; Callegari, Federica; Todaro, Mary Antonio Donatello; Ardizzoni, Andrea; Rossini, Gian Paolo; Blasi, Elisabetta; Peppoloni, Samuele. - In: TOXICON. - ISSN 0041-0101. - STAMPA. - 55:2-3(2010), pp. 265-273. [10.1016/j.toxicon.2009.07.033]

Yessotoxin inhibits phagocytic activity of macrophages.

ORSI, Carlotta Francesca;COLOMBARI, Bruna;CALLEGARI, Federica;TODARO, Mary Antonio Donatello;ARDIZZONI, Andrea;ROSSINI, Gian Paolo;BLASI, Elisabetta;PEPPOLONI, Samuele
2010

Abstract

Yessotoxin (YTX) is a sulphated polyether compound produced by some species of dinoflagellate algae, that can be accumulated in bivalve mollusks and ingested by humans upon eating contaminated shellfish. Experiments in mice have demonstrated the lethal effect of YTX after intraperitoneal injection, whereas its oral administration has only limited acute toxicity, coupled with an alteration of plasma membrane protein turnover in the colon of the animals. In vitro studies have shown that this effect is due to the inhibition of endocytosis induced by the toxin. In this work, we investigated the effects of YTX on phagocytosis by using the J774 macrophage cell line. We found that macrophages exposed to 10 or 1nM YTX display a reduced phagocytic activity against Candida albicans; moreover, phagosome maturation is also inhibited in these cells. Such results were confirmed with resident peritoneal macrophages from normal mice. The inhibition of both phagocytosis and phagosome maturation likely involves cytoskeletal alterations, since a striking rearrangement of the F-actin organization occurs in YTX-treated J774 macrophages. Surprisingly, YTX also enhances cytokine production (TNF-alpha, MIP-1alpha and MIP-2) by J774 macrophages. Overall, our results show that low doses of YTX significantly affect both effector and secretory functions of macrophages.
2010
55
2-3
265
273
Yessotoxin inhibits phagocytic activity of macrophages / Orsi, Carlotta Francesca; Colombari, Bruna; Callegari, Federica; Todaro, Mary Antonio Donatello; Ardizzoni, Andrea; Rossini, Gian Paolo; Blasi, Elisabetta; Peppoloni, Samuele. - In: TOXICON. - ISSN 0041-0101. - STAMPA. - 55:2-3(2010), pp. 265-273. [10.1016/j.toxicon.2009.07.033]
Orsi, Carlotta Francesca; Colombari, Bruna; Callegari, Federica; Todaro, Mary Antonio Donatello; Ardizzoni, Andrea; Rossini, Gian Paolo; Blasi, Elisab...espandi
File in questo prodotto:
File Dimensione Formato  
Yessotoxin inhibits phagocytic activity of macrophages.pdf

Solo gestori archivio

Tipologia: Versione pubblicata dall'editore
Dimensione 974.82 kB
Formato Adobe PDF
974.82 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/618108
Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 21
social impact