The transcription factor NF-Y is a trimer with histone-likesubunits that binds and activates CCAAT-containingpromoters. NF-Y controls the expressionof several key regulators of the cell cycle. In thisstudy, we examined the functional and moleculareffects of NF-YB knockdown. Cell cycle progressionis affected with a G2/M-specific depletion. This isdue to the inability of activation of G2/M-specificgenes, as evidenced by expression profiling, RT-PCRand ChIP data. Surprisingly, apoptosis is alsoobserved, with Caspase 3/7/8 cleavage. A role ofp53 and Bcl-2 family members is important. NF-YBinactivation is sufficient to functionally activate p53,in the absence of DNA damage. Failure to maintain aphysiologic level of CCAAT-dependent transcriptionof anti-apoptotic genes contributes to impairment ofBax/Bcl-2 and Bax/Bcl-XL ratios. Our data highlightthe importance of fine balancing the NF-Y-p53 duofor cell survival by (i) maintaining transcription ofanti-apoptotic genes and (ii) preventing p53 activationthat triggers the apoptotic cascade.
A balance between NF-Y and p53 governs the pro- and anti-apoptotic transcriptional response / Benatti, Paolo; Basile, Valentina; Merico, D; Fantoni, Luca Isaia; Tagliafico, Enrico; Imbriano, Carol. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 36 (5)(2008), pp. 1415-1428. [10.1093/nar/gkm1046]
Data di pubblicazione: | 2008 | |
Titolo: | A balance between NF-Y and p53 governs the pro- and anti-apoptotic transcriptional response | |
Autore/i: | Benatti, Paolo; Basile, Valentina; Merico, D; Fantoni, Luca Isaia; Tagliafico, Enrico; Imbriano, Carol | |
Autore/i UNIMORE: | ||
Digital Object Identifier (DOI): | http://dx.doi.org/10.1093/nar/gkm1046 | |
Rivista: | ||
Volume: | 36 (5) | |
Pagina iniziale: | 1415 | |
Pagina finale: | 1428 | |
Codice identificativo ISI: | WOS:000254294800010 | |
Codice identificativo Scopus: | 2-s2.0-41149094081 | |
Codice identificativo Pubmed: | 18187512 | |
Citazione: | A balance between NF-Y and p53 governs the pro- and anti-apoptotic transcriptional response / Benatti, Paolo; Basile, Valentina; Merico, D; Fantoni, Luca Isaia; Tagliafico, Enrico; Imbriano, Carol. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 36 (5)(2008), pp. 1415-1428. [10.1093/nar/gkm1046] | |
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Benatti et al., NAR 2008.pdf | Articolo principale | Versione dell'editore (versione pubblicata) | Open Access Visualizza/Apri |
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