Chromatin organization in the holocentric chromosomes of the aphid Schizaphis graminum has been investigated at a cytological level after C-banding, NOR, Giemsa, DAPI and CMA(3) staining. C-banding technique showed the presence of numerous C bands on the two X chromosomes both in telomeric and intercalary regions, whereas autosomes show a small number of heterochromatic bands. Contrary to the results with other aphid species, in S. graminum the C-banding pattern is peculiar to each chromosome pair, thus allowing the identification of homologues and the reliable reconstruction of a karyotype. These cytogenetic data could be useful for the identification of chromosomal rearrangement eventually occurred between different S. graminum biotypes. Moreover, silver staining and fluorescent in situ hybridization (FISH) with a 28S rDNA probe localized rDNA genes on one telomere of each X chromosome; these are the only brightly fluorescent C-positive regions revealed after CMA(3) staining, whereas all other heterochromatic bands are DAPI positive.

Cytogenetic analysis of the holocentric chromosomes of the aphid Schizaphis graminum / Mandrioli, Mauro; Ganassi, S; Bizzaro, D; Manicardi, Gian Carlo. - In: HEREDITAS. - ISSN 0018-0661. - STAMPA. - 131:(1999), pp. 185-190.

Cytogenetic analysis of the holocentric chromosomes of the aphid Schizaphis graminum

MANDRIOLI, Mauro;MANICARDI, Gian Carlo
1999

Abstract

Chromatin organization in the holocentric chromosomes of the aphid Schizaphis graminum has been investigated at a cytological level after C-banding, NOR, Giemsa, DAPI and CMA(3) staining. C-banding technique showed the presence of numerous C bands on the two X chromosomes both in telomeric and intercalary regions, whereas autosomes show a small number of heterochromatic bands. Contrary to the results with other aphid species, in S. graminum the C-banding pattern is peculiar to each chromosome pair, thus allowing the identification of homologues and the reliable reconstruction of a karyotype. These cytogenetic data could be useful for the identification of chromosomal rearrangement eventually occurred between different S. graminum biotypes. Moreover, silver staining and fluorescent in situ hybridization (FISH) with a 28S rDNA probe localized rDNA genes on one telomere of each X chromosome; these are the only brightly fluorescent C-positive regions revealed after CMA(3) staining, whereas all other heterochromatic bands are DAPI positive.
1999
131
185
190
Cytogenetic analysis of the holocentric chromosomes of the aphid Schizaphis graminum / Mandrioli, Mauro; Ganassi, S; Bizzaro, D; Manicardi, Gian Carlo. - In: HEREDITAS. - ISSN 0018-0661. - STAMPA. - 131:(1999), pp. 185-190.
Mandrioli, Mauro; Ganassi, S; Bizzaro, D; Manicardi, Gian Carlo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/613323
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