EnglishThe diagnosis of pleural tuberculosis (plTB) by the analysis of pleural effusions (PEs) with standard diagnostic tools is difficult. In routine clinical practice, the present authors evaluated the performance of a commercially available Mycobacterium tuberculosis (MTB)-specific enzyme-linked immunospot assay on peripheral blood mononuclear cells (PBMCs) and pleural effusion mononuclear cells (PEMCs) in patients with suspect plTB. The T-SPOT.TB test (Oxford Immunotec Ltd, Abingdon, UK) was performed on PBMCs and PEMCs in 20 patients with a clinical and radiological suspect of plTB and in 21 control subjects with a diagnosis of PE of nontuberculous origin at four centres participating in the European Tuberculosis Network. In total, 18 (90%) out of 20 patients with plTB tested T-SPOT.TB-positive on PBMCs and 19 (95%) out of 20 on PEMCs. Among controls, T-SPOT.TB was positive in seven out of 21 (33%) patients when performed on PBMCs (these patients were assumed to be latently infected with MTB) and five (23%) out of 21 when performed on PEMCs. Sensitivity and specificity of T-SPOT.TB for the diagnosis of active plTB when performed on PEMCs were 95 and 76%, respectively. Enumerating Mycobacterium tuberculosis-specific T-cells in pleural effusion mononuclear cells by ELISPOT is feasible in routine clinical practice and may be useful for a rapid and accurate diagnosis of pleural tuberculosis.
| Data di pubblicazione: | 2007 |
| Titolo: | Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy. |
| Autore/i: | M. Losi; A. Bossink; L. Codecasa; C. Jafari; M. Ernst; S. Thijsen; D. Cirillo; M. Ferrarese; U. Greinert; L. M. Fabbri; L. Richeldi; C. Lange; European Tuberculosis Network |
| Autore/i UNIMORE: | |
| Rivista: | |
| Volume: | 30 |
| Pagina iniziale: | 1173 |
| Pagina finale: | 1179 |
| Codice identificativo ISI: | WOS:000251521800023 |
| Codice identificativo Scopus: | 2-s2.0-38849095318 |
| Codice identificativo Pubmed: | 17715165 |
| Citazione: | Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy / M. Losi; A. Bossink; L. Codecasa; C. Jafari; M. Ernst; S. Thijsen; D. Cirillo; M. Ferrarese; U. Greinert; L. M. Fabbri; L. Richeldi; C. Lange; European Tuberculosis Network. - In: EUROPEAN RESPIRATORY JOURNAL. - ISSN 0903-1936. - STAMPA. - 30(2007), pp. 1173-1179. |
| Tipologia | Articolo su rivista |
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