The diagnosis of pleural tuberculosis (plTB) by the analysis of pleural effusions (PEs) with standard diagnostic tools is difficult. In routine clinical practice, the present authors evaluated the performance of a commercially available Mycobacterium tuberculosis (MTB)-specific enzyme-linked immunospot assay on peripheral blood mononuclear cells (PBMCs) and pleural effusion mononuclear cells (PEMCs) in patients with suspect plTB. The T-SPOT.TB test (Oxford Immunotec Ltd, Abingdon, UK) was performed on PBMCs and PEMCs in 20 patients with a clinical and radiological suspect of plTB and in 21 control subjects with a diagnosis of PE of nontuberculous origin at four centres participating in the European Tuberculosis Network. In total, 18 (90%) out of 20 patients with plTB tested T-SPOT.TB-positive on PBMCs and 19 (95%) out of 20 on PEMCs. Among controls, T-SPOT.TB was positive in seven out of 21 (33%) patients when performed on PBMCs (these patients were assumed to be latently infected with MTB) and five (23%) out of 21 when performed on PEMCs. Sensitivity and specificity of T-SPOT.TB for the diagnosis of active plTB when performed on PEMCs were 95 and 76%, respectively. Enumerating Mycobacterium tuberculosis-specific T-cells in pleural effusion mononuclear cells by ELISPOT is feasible in routine clinical practice and may be useful for a rapid and accurate diagnosis of pleural tuberculosis.
|Data di pubblicazione:||2007|
|Titolo:||Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy.|
|Autore/i:||M. Losi; A. Bossink; L. Codecasa; C. Jafari; M. Ernst; S. Thijsen; D. Cirillo; M. Ferrarese; U. Greinert; L. M. Fabbri; L. Richeldi; C. Lange; European Tuberculosis Network|
|Codice identificativo ISI:||WOS:000251521800023|
|Codice identificativo Scopus:||2-s2.0-38849095318|
|Codice identificativo Pubmed:||17715165|
|Citazione:||Use of a T-cell interferon-gamma release assay for the diagnosis of tuberculous pleurisy / M. Losi; A. Bossink; L. Codecasa; C. Jafari; M. Ernst; S. Thijsen; D. Cirillo; M. Ferrarese; U. Greinert; L. M. Fabbri; L. Richeldi; C. Lange; European Tuberculosis Network. - In: EUROPEAN RESPIRATORY JOURNAL. - ISSN 0903-1936. - STAMPA. - 30(2007), pp. 1173-1179.|
|Tipologia||Articolo su rivista|
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