Cellular localization of nanoparticles (Np) represents an important target in the understanding of their distribution after endovenous injection. The need of suitable devices and methodologies capable to detect Np in tissues or in cellular districts can be satisfied by Np which have to be easily recognizable by simple methods. Conjugations of poly(D,L-lactide-co-glycolide) with fluorescein and biotin allow fluorescent and immuno-histochemically active Np to be obtained. The fluorescein Np are detectable using fluorescent microscopy whereas biotin Np can be detected by optical microscopy after streptavidin-biotin-peroxidase complexation. In vivo experiments confirm the ability of these particles to be easily detected in the brain parenchyma or in the liver cell population according to the infusion pathway.

Conjugated poly(D,L-lactide-co-glycolide) for the preparation of in vivo detectable nanoparticles / Tosi, Giovanni; Rivasi, Francesco; F., Gandolfi; Costantino, Luca; Vandelli, Maria Angela; Forni, Flavio. - In: BIOMATERIALS. - ISSN 0142-9612. - STAMPA. - 26:(2005), pp. 4189-4195. [10.1016/biomaterials.2004.10.025]

Conjugated poly(D,L-lactide-co-glycolide) for the preparation of in vivo detectable nanoparticles

TOSI, Giovanni;RIVASI, Francesco;COSTANTINO, Luca;VANDELLI, Maria Angela;FORNI, Flavio
2005

Abstract

Cellular localization of nanoparticles (Np) represents an important target in the understanding of their distribution after endovenous injection. The need of suitable devices and methodologies capable to detect Np in tissues or in cellular districts can be satisfied by Np which have to be easily recognizable by simple methods. Conjugations of poly(D,L-lactide-co-glycolide) with fluorescein and biotin allow fluorescent and immuno-histochemically active Np to be obtained. The fluorescein Np are detectable using fluorescent microscopy whereas biotin Np can be detected by optical microscopy after streptavidin-biotin-peroxidase complexation. In vivo experiments confirm the ability of these particles to be easily detected in the brain parenchyma or in the liver cell population according to the infusion pathway.
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4189
4195
Conjugated poly(D,L-lactide-co-glycolide) for the preparation of in vivo detectable nanoparticles / Tosi, Giovanni; Rivasi, Francesco; F., Gandolfi; Costantino, Luca; Vandelli, Maria Angela; Forni, Flavio. - In: BIOMATERIALS. - ISSN 0142-9612. - STAMPA. - 26:(2005), pp. 4189-4195. [10.1016/biomaterials.2004.10.025]
Tosi, Giovanni; Rivasi, Francesco; F., Gandolfi; Costantino, Luca; Vandelli, Maria Angela; Forni, Flavio
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/612771
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