Response surface methodology (RSM) was applied in optimizing the production of volatile organic compounds (VOCs) by shake-flask cultures of a selected Trichosporon moniliiforme strain (DBVPG 4665). A factorial experiment (2(3)) was designed by setting three parameters at two levels: X-1 = incubation time (IT = 72-168 h); X-2 = pH (4-7); X-3 = L-methionine concentration (L-MET = 5-20 g l(-1)). On the basis of the results of factorial design, X-2 (pH) and X-3 (L-MET) were optimized by performing a central composite design (CCD). Second-order polynomial models and response surfaces were obtained for total VOC concentration (VOC1) and for the percentage of dimethyl disulphide (DMDS)/total VOCs (%DMDS). A range of variation of X-2 and X-3 able to maximize simultaneously both responses has been found. Average final values of responses (VOC1 = 4.9 +/- 1.3 mg l(-1); %DMDS = 97.6 +/- 1.1%) were about 532 and 133% of data of central point and were in good agreement with calculated values, thus confirming the reliability of our empirical model in optimizing VOCs production by T. moniliiforme (c) 2006 Elsevier Inc. All rights reserved.

Application of the response surface methodology (RSM) for optimizing the production of volatile organic compounds (VOCs) by Trichosporon moniliiforme / C., Gasparetti; P., Buzzini; Cramarossa, Maria Rita; B., Turchetti; Pagnoni, Ugo Maria; Forti, Luca. - In: ENZYME AND MICROBIAL TECHNOLOGY. - ISSN 0141-0229. - STAMPA. - 39:6(2006), pp. 1341-1346. [10.1016/j.enzmictec.2006.04.016]

Application of the response surface methodology (RSM) for optimizing the production of volatile organic compounds (VOCs) by Trichosporon moniliiforme

CRAMAROSSA, Maria Rita;PAGNONI, Ugo Maria;FORTI, Luca
2006

Abstract

Response surface methodology (RSM) was applied in optimizing the production of volatile organic compounds (VOCs) by shake-flask cultures of a selected Trichosporon moniliiforme strain (DBVPG 4665). A factorial experiment (2(3)) was designed by setting three parameters at two levels: X-1 = incubation time (IT = 72-168 h); X-2 = pH (4-7); X-3 = L-methionine concentration (L-MET = 5-20 g l(-1)). On the basis of the results of factorial design, X-2 (pH) and X-3 (L-MET) were optimized by performing a central composite design (CCD). Second-order polynomial models and response surfaces were obtained for total VOC concentration (VOC1) and for the percentage of dimethyl disulphide (DMDS)/total VOCs (%DMDS). A range of variation of X-2 and X-3 able to maximize simultaneously both responses has been found. Average final values of responses (VOC1 = 4.9 +/- 1.3 mg l(-1); %DMDS = 97.6 +/- 1.1%) were about 532 and 133% of data of central point and were in good agreement with calculated values, thus confirming the reliability of our empirical model in optimizing VOCs production by T. moniliiforme (c) 2006 Elsevier Inc. All rights reserved.
2006
39
6
1341
1346
Application of the response surface methodology (RSM) for optimizing the production of volatile organic compounds (VOCs) by Trichosporon moniliiforme / C., Gasparetti; P., Buzzini; Cramarossa, Maria Rita; B., Turchetti; Pagnoni, Ugo Maria; Forti, Luca. - In: ENZYME AND MICROBIAL TECHNOLOGY. - ISSN 0141-0229. - STAMPA. - 39:6(2006), pp. 1341-1346. [10.1016/j.enzmictec.2006.04.016]
C., Gasparetti; P., Buzzini; Cramarossa, Maria Rita; B., Turchetti; Pagnoni, Ugo Maria; Forti, Luca
File in questo prodotto:
File Dimensione Formato  
43_Application of the response surface methodology (RSM) for optimizing the production of volatile organic compounds (VOCs) by Trichosporon moniliiforme.pdf

Accesso riservato

Tipologia: Versione dell'autore revisionata e accettata per la pubblicazione
Dimensione 237.98 kB
Formato Adobe PDF
237.98 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/612581
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 7
  • ???jsp.display-item.citation.isi??? 7
social impact