Chromatin organization in the holocentric chromosomes of the aphid Brevicoryne brassicae has been investigated at a cytological level after C-banding, NOR, Giemsa, DAPI and CMA(3) staining. C-banding technique showed the presence of heterochromatic bands on both telomeres of the two X chromosomes, whereas only the longest pair of autosomes show distinct intercalary C-positive bands. Moreover, silver staining and fluorescent in situ hybridization (FISH) with a 28S rDNA probe localized rDNA genes on one telomere of each X chromosome; these are the only brightly fluorescent C-positive regions revealed after CMA(3) staining, whereas all other heterochromatic bands are DAPI positive.

Cytogenetic analysis on the holocentric chromosomes of the cabbage aphid Brevicoryne brassicae / Manicardi, Gian Carlo; Mandrioli, Mauro. - In: CARYOLOGIA. - ISSN 0008-7114. - STAMPA. - 56:2(2003), pp. 143-147. [10.1080/00087114.2003.10589317]

Cytogenetic analysis on the holocentric chromosomes of the cabbage aphid Brevicoryne brassicae

MANICARDI, Gian Carlo;MANDRIOLI, Mauro
2003

Abstract

Chromatin organization in the holocentric chromosomes of the aphid Brevicoryne brassicae has been investigated at a cytological level after C-banding, NOR, Giemsa, DAPI and CMA(3) staining. C-banding technique showed the presence of heterochromatic bands on both telomeres of the two X chromosomes, whereas only the longest pair of autosomes show distinct intercalary C-positive bands. Moreover, silver staining and fluorescent in situ hybridization (FISH) with a 28S rDNA probe localized rDNA genes on one telomere of each X chromosome; these are the only brightly fluorescent C-positive regions revealed after CMA(3) staining, whereas all other heterochromatic bands are DAPI positive.
2003
56
2
143
147
Cytogenetic analysis on the holocentric chromosomes of the cabbage aphid Brevicoryne brassicae / Manicardi, Gian Carlo; Mandrioli, Mauro. - In: CARYOLOGIA. - ISSN 0008-7114. - STAMPA. - 56:2(2003), pp. 143-147. [10.1080/00087114.2003.10589317]
Manicardi, Gian Carlo; Mandrioli, Mauro
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/612333
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