Increased number of peripheral benzodiazepine receptors (PBRs) have been found in some tumors outside the liver. The present study was to verify whether the PBR system is altered in hepatocellular carcinoma (HCC). The levels of endogenous benzodiazepine-like compounds (BZDs). measured by radioreceptor binding technique after HPLC purification and the endogenous ligand for PBRs. termed diazepam binding inhibitor (DBI). measured by radioimmunoassay utilizing a specific antibody for human DBI, were studied in the blood of 15 normal subjects, 12 liver cirrhosis and 10 patients with HCC. The levels of BZDs in serum were increased hundred fold in liver cirrhosis patients and slightly elevated in HCC patients. DBI was found to be increased in HCC patients. The binding recognition sites for PBRs (Bmax) were increased 4 to 7 fold in HCC tissue in comparison with that found in non-tumoral liver tissue (NTLT). On the contrary the concentrations of DBI were found to be significantly decreased in HCC tissue in comparison with the respective NTLT. These results seem to suggest an implication of PBRs and of their putative endogenous ligands in the metabolism of these neoplastic cells and possibly in their proliferation. The up-regulation of PBRs found in HCC tissue seems to indicate an increased functional activity of these receptors and opens up the possibility of new pharmacological and diagnostic approaches while the changes in the circulating endogenous ligands for the above receptors might be envisaged as early markers of tumorigenesis in liver cirrhosis.

Up-regulation of peripheral benzodiazepine receptors system in hepatocellular carcinoma / Venturini, I; Zeneroli, Maria Luisa; Corsi, Lorenzo; Avallone, Rossella; Farina, Franco; Alho, H; Baraldi, C; Pecora, N; Frigo, M; Ardizzone, G; Arrigo, A; Pellicci, R; Baraldi, Mario. - In: LIFE SCIENCES. - ISSN 0024-3205. - ELETTRONICO. - 63:(1998), pp. 1269-1280.

Up-regulation of peripheral benzodiazepine receptors system in hepatocellular carcinoma.

ZENEROLI, Maria Luisa;CORSI, Lorenzo;AVALLONE, Rossella;FARINA, Franco;BARALDI, Mario
1998

Abstract

Increased number of peripheral benzodiazepine receptors (PBRs) have been found in some tumors outside the liver. The present study was to verify whether the PBR system is altered in hepatocellular carcinoma (HCC). The levels of endogenous benzodiazepine-like compounds (BZDs). measured by radioreceptor binding technique after HPLC purification and the endogenous ligand for PBRs. termed diazepam binding inhibitor (DBI). measured by radioimmunoassay utilizing a specific antibody for human DBI, were studied in the blood of 15 normal subjects, 12 liver cirrhosis and 10 patients with HCC. The levels of BZDs in serum were increased hundred fold in liver cirrhosis patients and slightly elevated in HCC patients. DBI was found to be increased in HCC patients. The binding recognition sites for PBRs (Bmax) were increased 4 to 7 fold in HCC tissue in comparison with that found in non-tumoral liver tissue (NTLT). On the contrary the concentrations of DBI were found to be significantly decreased in HCC tissue in comparison with the respective NTLT. These results seem to suggest an implication of PBRs and of their putative endogenous ligands in the metabolism of these neoplastic cells and possibly in their proliferation. The up-regulation of PBRs found in HCC tissue seems to indicate an increased functional activity of these receptors and opens up the possibility of new pharmacological and diagnostic approaches while the changes in the circulating endogenous ligands for the above receptors might be envisaged as early markers of tumorigenesis in liver cirrhosis.
63
1269
1280
Up-regulation of peripheral benzodiazepine receptors system in hepatocellular carcinoma / Venturini, I; Zeneroli, Maria Luisa; Corsi, Lorenzo; Avallone, Rossella; Farina, Franco; Alho, H; Baraldi, C; Pecora, N; Frigo, M; Ardizzone, G; Arrigo, A; Pellicci, R; Baraldi, Mario. - In: LIFE SCIENCES. - ISSN 0024-3205. - ELETTRONICO. - 63:(1998), pp. 1269-1280.
Venturini, I; Zeneroli, Maria Luisa; Corsi, Lorenzo; Avallone, Rossella; Farina, Franco; Alho, H; Baraldi, C; Pecora, N; Frigo, M; Ardizzone, G; Arrigo, A; Pellicci, R; Baraldi, Mario
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/612183
Citazioni
  • ???jsp.display-item.citation.pmc??? 13
  • Scopus 68
  • ???jsp.display-item.citation.isi??? 64
social impact