Chromatin organization in the holocentric chromosomes of the green apple aphid Aphis pomi has been investigated at a cytological level after C-banding, NOR, Giemsa, fluorochrome staining and fluorescent in situ hybridization (FISH). C-banding technique showed that heterochromatic bands are exclusively located on X chromosomes. This data represents a peculiar feature that clearly contradicts the equilocal distribution of heterochromatin typical of monocentric chromosomes. Moreover, silver staining and FISH carried out with a 28S rDNA probe localized rDNA genes on one telomere of each X chromosome; CMA(3) staining reveals that these silver positive telomeres are, the only GC-rich regions among A. pomi heterochromatin, whereas all other C-positive bands are DAPI positive thus containing AT-rich DNA.

X-linked heterochromatin distribution in the holocentric chromosomes of the green apple aphid Aphis pomi / A., Criniti; G., Simonazzi; Cassanelli, Stefano; M., Ferrari; D., Bizzaro; Manicardi, Gian Carlo. - In: GENETICA. - ISSN 0016-6707. - ELETTRONICO. - 124:(2005), pp. 93-98.

X-linked heterochromatin distribution in the holocentric chromosomes of the green apple aphid Aphis pomi

CASSANELLI, Stefano;MANICARDI, Gian Carlo
2005-01-01

Abstract

Chromatin organization in the holocentric chromosomes of the green apple aphid Aphis pomi has been investigated at a cytological level after C-banding, NOR, Giemsa, fluorochrome staining and fluorescent in situ hybridization (FISH). C-banding technique showed that heterochromatic bands are exclusively located on X chromosomes. This data represents a peculiar feature that clearly contradicts the equilocal distribution of heterochromatin typical of monocentric chromosomes. Moreover, silver staining and FISH carried out with a 28S rDNA probe localized rDNA genes on one telomere of each X chromosome; CMA(3) staining reveals that these silver positive telomeres are, the only GC-rich regions among A. pomi heterochromatin, whereas all other C-positive bands are DAPI positive thus containing AT-rich DNA.
124
93
98
X-linked heterochromatin distribution in the holocentric chromosomes of the green apple aphid Aphis pomi / A., Criniti; G., Simonazzi; Cassanelli, Stefano; M., Ferrari; D., Bizzaro; Manicardi, Gian Carlo. - In: GENETICA. - ISSN 0016-6707. - ELETTRONICO. - 124:(2005), pp. 93-98.
A., Criniti; G., Simonazzi; Cassanelli, Stefano; M., Ferrari; D., Bizzaro; Manicardi, Gian Carlo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/611907
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