potential clinical application, we have investigated whether zinc-containing 45S5 (HZ) glasses might be cytotoxic for human rs on Bioactive glasses such as Hench’s 45S5 have applications to tissue engineering and bone repair: the insertion of zinc has been proposed to improve their bone-bonding ability and to slacken their dissolution in extracellular body fluids. In view of a MG-63 osteoblasts. In our experimental conditions, after 24 h of incubation HZ glasses released significant amounts of Zn2+ and induced in MG-63 cells release of lactate dehydrogenase (index of cytotoxicity) and the following indexes of oxidative stress: (i) accumulation of intracellular malonyldialdehyde, (ii) increased activity of pentose phosphate pathway, (iii) increased expression of peheme oxygenase-1, (iv) increased activity of Cu,Zn-superoxide dismutase, (v) decreased level of intracellular thiols. These effects were inversely related to the zinc content of glass powders, were mimicked by ZnCl2 solutions and were prevented by either metal chelators (EDTA, NTA) or the antioxidant ascorbate, suggesting that Zn2+ released fastly from HZ glasses can cause MG-63 cell damage via an oxidative stress. This work highlights the importance of designing Zn-containing bioactive glasses without cytotoxic effects and gives supplementary information about the prooxidant role of zinc in living systems.

Cytoxicity of zinc-cotaining bioactive glasses in contact with human osteoblasts / V., Aina; A., Perardi; L., Bergiandi; Malavasi, Gianluca; Menabue, Ledi; C., Morterra; D., Ghigo. - In: CHEMICO-BIOLOGICAL INTERACTIONS. - ISSN 0009-2797. - ELETTRONICO. - 167:3(2007), pp. 207-218. [10.1016/j.cbi.2007.03.002]

Cytoxicity of zinc-cotaining bioactive glasses in contact with human osteoblasts

MALAVASI, Gianluca;MENABUE, Ledi;
2007

Abstract

potential clinical application, we have investigated whether zinc-containing 45S5 (HZ) glasses might be cytotoxic for human rs on Bioactive glasses such as Hench’s 45S5 have applications to tissue engineering and bone repair: the insertion of zinc has been proposed to improve their bone-bonding ability and to slacken their dissolution in extracellular body fluids. In view of a MG-63 osteoblasts. In our experimental conditions, after 24 h of incubation HZ glasses released significant amounts of Zn2+ and induced in MG-63 cells release of lactate dehydrogenase (index of cytotoxicity) and the following indexes of oxidative stress: (i) accumulation of intracellular malonyldialdehyde, (ii) increased activity of pentose phosphate pathway, (iii) increased expression of peheme oxygenase-1, (iv) increased activity of Cu,Zn-superoxide dismutase, (v) decreased level of intracellular thiols. These effects were inversely related to the zinc content of glass powders, were mimicked by ZnCl2 solutions and were prevented by either metal chelators (EDTA, NTA) or the antioxidant ascorbate, suggesting that Zn2+ released fastly from HZ glasses can cause MG-63 cell damage via an oxidative stress. This work highlights the importance of designing Zn-containing bioactive glasses without cytotoxic effects and gives supplementary information about the prooxidant role of zinc in living systems.
2007
167
3
207
218
Cytoxicity of zinc-cotaining bioactive glasses in contact with human osteoblasts / V., Aina; A., Perardi; L., Bergiandi; Malavasi, Gianluca; Menabue, Ledi; C., Morterra; D., Ghigo. - In: CHEMICO-BIOLOGICAL INTERACTIONS. - ISSN 0009-2797. - ELETTRONICO. - 167:3(2007), pp. 207-218. [10.1016/j.cbi.2007.03.002]
V., Aina; A., Perardi; L., Bergiandi; Malavasi, Gianluca; Menabue, Ledi; C., Morterra; D., Ghigo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/611779
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