We report the complete separation and characterization by online high-performance liquidchromatography-electrospray ionization mass spectrometry (LC-ESI-MS) of fully saturatedalginic acid (AA) oligosaccharides from DP1 to beyond DP23, obtained by a chemicalprocess, and unsaturated oligomers from DP1 to DP10, produced by lyase treatment. Aseries of negatively charged species of different m/z ratio are seen for each oligosaccharide.Smaller AA species, from DP1 to DP4, mainly furnish [M–H]- anions whereas the DP5 toDP9-10 oligomers predominantly exist as the 2- charge state. The AA oligomers from DP10to DP17 are mainly represented by the [M–3H]3- anions whereas species from DP18 toDP23 are characterized by the 4- charge state. Online LC-ESI-MS enabled separation andsimultaneous characterization of complex saturated and unsaturated AA oligomer mixtureswithout previous sample treatment, in particular extensive removal of salts to obtain speciescompatible with ESI-MS.

HPLC separation and on-line MS characterization of saturated and unsaturated alginic acid oligomers / Volpi, Nicola; Maccari, Francesca. - In: CHROMATOGRAPHIA. - ISSN 0009-5893. - STAMPA. - 69(2009), pp. 813-819.

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