We obtained affinity-purified polyclonal anti-id antibodies against mAb MhC1 and BrhC3, which recognize amino acids 133-147 at the N-terminus of mature human IL-1 beta. mAb MhC1 and BrhC3 have been shown to inhibit binding of IL-1 beta to type I IL-1R, and to neutralize IL-1 beta bioactivity in a number of in vitro assays. We show that affinity-purified antibodies against the MhC1 and BrhC3 idiotypes specifically bind to type I IL-1 beta IL-1R and that this binding is inhibited by both IL-1 beta and IL-1ra; anti-id antibodies were also able to trigger IL-1R-dependent events, such as IL-8 secretion by human skin fibroblasts and pyrogenic effect after injection in mice. These anti-id antibodies, therefore, behave as structural and functional "images" of IL-1 beta, both in vivo and in vitro. These data indicate the idiotypic strategy as a powerful tool to study the fine specificity of receptor-ligand interactions. Moreover, this is, to our knowledge, the first report showing that the "internal image" of a cytokine can be active in vivo

Anti-idiotypic antibodies mimick structural and functional properties of IL-1β in its interaction with type I IL-1R / Soprano, E; Vogo, E; Verani, A; Blom, Johanna Maria Catharina; Siccardi, A; Vercelli, D. VIALE G.. - In: LYMPHOKINE AND CYTOKINE RESEARCH. - ISSN 1056-5477. - STAMPA. - 13:(1994), pp. 325-329.

Anti-idiotypic antibodies mimick structural and functional properties of IL-1β in its interaction with type I IL-1R.

BLOM, Johanna Maria Catharina;
1994

Abstract

We obtained affinity-purified polyclonal anti-id antibodies against mAb MhC1 and BrhC3, which recognize amino acids 133-147 at the N-terminus of mature human IL-1 beta. mAb MhC1 and BrhC3 have been shown to inhibit binding of IL-1 beta to type I IL-1R, and to neutralize IL-1 beta bioactivity in a number of in vitro assays. We show that affinity-purified antibodies against the MhC1 and BrhC3 idiotypes specifically bind to type I IL-1 beta IL-1R and that this binding is inhibited by both IL-1 beta and IL-1ra; anti-id antibodies were also able to trigger IL-1R-dependent events, such as IL-8 secretion by human skin fibroblasts and pyrogenic effect after injection in mice. These anti-id antibodies, therefore, behave as structural and functional "images" of IL-1 beta, both in vivo and in vitro. These data indicate the idiotypic strategy as a powerful tool to study the fine specificity of receptor-ligand interactions. Moreover, this is, to our knowledge, the first report showing that the "internal image" of a cytokine can be active in vivo
1994
13
325
329
Anti-idiotypic antibodies mimick structural and functional properties of IL-1β in its interaction with type I IL-1R / Soprano, E; Vogo, E; Verani, A; Blom, Johanna Maria Catharina; Siccardi, A; Vercelli, D. VIALE G.. - In: LYMPHOKINE AND CYTOKINE RESEARCH. - ISSN 1056-5477. - STAMPA. - 13:(1994), pp. 325-329.
Soprano, E; Vogo, E; Verani, A; Blom, Johanna Maria Catharina; Siccardi, A; Vercelli, D. VIALE G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/607940
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