Supported lipid bilayers (SLB) composed of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE) and -phosphatidylglycerol (POPG) were assembled by the vesicle fusion technique on mica and studied by temperature-controlled AFM. The role of different physical parameters on the main phase transition was elucidated. Both mixed (POPE/POPG 3:1) and pure POPE bilayers were studied. By increasing the ionic strength of the solution and the incubation temperature, a shift from a decoupled phase transition of the two leaflets, to a coupled transition, with domains in register, was obtained. The observed behavior points to a modulation of the substrate/bilayer and interleaflet coupling induced by the environment and preparation conditions of supported lipid bilayers. The results are discussed in view of the role of different interactions in the system. The influence of the substrate on the lipid bilayers can help understanding the possible effect that submembrane elements like the cytoskeleton might have on the structure and dynamics of biomembranes also in terms of interleaflet coupling.
Effect of Physical Parameters on the Main Phase Transition of Supported Lipid Bilayers / H. M., Seeger; G., Marino; Alessandrini, Andrea; P., Facci. - In: BIOPHYSICAL JOURNAL. - ISSN 0006-3495. - STAMPA. - 97:4(2009), pp. 1067-1076. [10.1016/j.bpj.2009.03.068]
Effect of Physical Parameters on the Main Phase Transition of Supported Lipid Bilayers
ALESSANDRINI, Andrea;
2009
Abstract
Supported lipid bilayers (SLB) composed of 1-palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE) and -phosphatidylglycerol (POPG) were assembled by the vesicle fusion technique on mica and studied by temperature-controlled AFM. The role of different physical parameters on the main phase transition was elucidated. Both mixed (POPE/POPG 3:1) and pure POPE bilayers were studied. By increasing the ionic strength of the solution and the incubation temperature, a shift from a decoupled phase transition of the two leaflets, to a coupled transition, with domains in register, was obtained. The observed behavior points to a modulation of the substrate/bilayer and interleaflet coupling induced by the environment and preparation conditions of supported lipid bilayers. The results are discussed in view of the role of different interactions in the system. The influence of the substrate on the lipid bilayers can help understanding the possible effect that submembrane elements like the cytoskeleton might have on the structure and dynamics of biomembranes also in terms of interleaflet coupling.
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