The green peach aphid (Myzus persicae), is lastly one of well studied insect model todissect insecticide resistance mechanisms. As a result, a few in-vitro diagnostic tools were added to thetraditional in-vivo bioassays to monitor the presence of resistance in populations of this pest. Sincedifferent control strategies and different resistance mechanisms are often involved, both biochemicaland molecular approaches must be followed. Biochemical assays can be used to asses esterasedetoxifying/sequestering activity as well as the acetylcholinesterase insensitivity to some insecticides.Molecular screening by RFLP-PCR, may be also effectively performed to detect previously identifiedtarget site mutations. Herewith we analyzed aphid populations collected in peach orchards fromEmilia-Romagna (the most important peach growing area in Italy) for the presence of elevated esteraseexpression and acetylcholinesterase insensitivity to pirmicarb. Gene mutation occurrence inacetylcholinesterase (S431F) and in voltage-dependent sodium channel (kdr), conferring resistance todimethylcarbammates.and pyretroids respectively, have been also.checked. Information regarding theco-selection of different insecticide resistance mechanisms are thought supporting technical assistanceservices, to select the most suitable pest management strategies against M. persicae.

Survey on the presence of “MACE” and “kdr” mutations in populations of the green peach aphid (Myzus persicae) from Emilia-Romagna peach orchards: preliminary results / Pavesi, F; Criniti, A; Cassanelli, Stefano; Bizzaro, D; Manicardi, Gian Carlo; Cravedi, P; Mazzoni, E.. - STAMPA. - 37:(2008), pp. 29-33. (Intervento presentato al convegno Integrated Plant Protection in Stone Fruit IOBC/wprs Bulletin, tenutosi a . nel .).

Survey on the presence of “MACE” and “kdr” mutations in populations of the green peach aphid (Myzus persicae) from Emilia-Romagna peach orchards: preliminary results

CASSANELLI, Stefano;MANICARDI, Gian Carlo;
2008

Abstract

The green peach aphid (Myzus persicae), is lastly one of well studied insect model todissect insecticide resistance mechanisms. As a result, a few in-vitro diagnostic tools were added to thetraditional in-vivo bioassays to monitor the presence of resistance in populations of this pest. Sincedifferent control strategies and different resistance mechanisms are often involved, both biochemicaland molecular approaches must be followed. Biochemical assays can be used to asses esterasedetoxifying/sequestering activity as well as the acetylcholinesterase insensitivity to some insecticides.Molecular screening by RFLP-PCR, may be also effectively performed to detect previously identifiedtarget site mutations. Herewith we analyzed aphid populations collected in peach orchards fromEmilia-Romagna (the most important peach growing area in Italy) for the presence of elevated esteraseexpression and acetylcholinesterase insensitivity to pirmicarb. Gene mutation occurrence inacetylcholinesterase (S431F) and in voltage-dependent sodium channel (kdr), conferring resistance todimethylcarbammates.and pyretroids respectively, have been also.checked. Information regarding theco-selection of different insecticide resistance mechanisms are thought supporting technical assistanceservices, to select the most suitable pest management strategies against M. persicae.
2008
Integrated Plant Protection in Stone Fruit IOBC/wprs Bulletin,
.
.
37
29
33
Pavesi, F; Criniti, A; Cassanelli, Stefano; Bizzaro, D; Manicardi, Gian Carlo; Cravedi, P; Mazzoni, E.
Survey on the presence of “MACE” and “kdr” mutations in populations of the green peach aphid (Myzus persicae) from Emilia-Romagna peach orchards: preliminary results / Pavesi, F; Criniti, A; Cassanelli, Stefano; Bizzaro, D; Manicardi, Gian Carlo; Cravedi, P; Mazzoni, E.. - STAMPA. - 37:(2008), pp. 29-33. (Intervento presentato al convegno Integrated Plant Protection in Stone Fruit IOBC/wprs Bulletin, tenutosi a . nel .).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/596665
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