We have established two murine cell lines derived from Small Cell Lung Carcinomas (SCLCs) developed by HPV-E6/E7 transgenic mice. These cells named PPAP-9 and PPAP-10 were isolated from mice bearing tumors, 9 and 10 months old, respectively. The cells, 5 mu m in diameter, express HPV oncoproteins and sustain tumor formation after subcutaneous injection in syngenic mice. A detailed analysis indicated the epithelial origin and the neuroendocrine differentiation of these cells. We showed by confocal immunofluorescence the expression of the epithelial marker cytokeratin 5, whose gene promoter was used to direct the expression of HPV E6/E. Cells express several neuroendocrine markers such as CGRP, MAP-2, Ash 1, CgrA, Scg2. The neuroendocrine differentiation of these cells was further confirmed by electron microscopy demonstrating neuropeptides secreting granules in their cytoplasm. Furthermore, in agreement with the altered expression observed in the majority of human SCLC we showed in these cells the absence of both p53 and pRB and a dramatic reduction in the expression of Caveolin-1. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

Establishment and characterization of murine small cell lung carcinoma cell lines derived from HPV-16 E6/E7 transgenic mice / L., Carraresi; R., Martinelli; A., Vannoni; Riccio, Massimo; Dembic, Maja; S., Tripodi; M., Cintorino; S., Santi; E., Bigliardi; M., Carmellini; M., Rossini. - In: CANCER LETTERS. - ISSN 0304-3835. - STAMPA. - 231:1(2006), pp. 65-73. [10.1016/j.canlet.2005.01.027]

Establishment and characterization of murine small cell lung carcinoma cell lines derived from HPV-16 E6/E7 transgenic mice

RICCIO, Massimo;DEMBIC, Maja;
2006

Abstract

We have established two murine cell lines derived from Small Cell Lung Carcinomas (SCLCs) developed by HPV-E6/E7 transgenic mice. These cells named PPAP-9 and PPAP-10 were isolated from mice bearing tumors, 9 and 10 months old, respectively. The cells, 5 mu m in diameter, express HPV oncoproteins and sustain tumor formation after subcutaneous injection in syngenic mice. A detailed analysis indicated the epithelial origin and the neuroendocrine differentiation of these cells. We showed by confocal immunofluorescence the expression of the epithelial marker cytokeratin 5, whose gene promoter was used to direct the expression of HPV E6/E. Cells express several neuroendocrine markers such as CGRP, MAP-2, Ash 1, CgrA, Scg2. The neuroendocrine differentiation of these cells was further confirmed by electron microscopy demonstrating neuropeptides secreting granules in their cytoplasm. Furthermore, in agreement with the altered expression observed in the majority of human SCLC we showed in these cells the absence of both p53 and pRB and a dramatic reduction in the expression of Caveolin-1. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
2006
231
1
65
73
Establishment and characterization of murine small cell lung carcinoma cell lines derived from HPV-16 E6/E7 transgenic mice / L., Carraresi; R., Martinelli; A., Vannoni; Riccio, Massimo; Dembic, Maja; S., Tripodi; M., Cintorino; S., Santi; E., Bigliardi; M., Carmellini; M., Rossini. - In: CANCER LETTERS. - ISSN 0304-3835. - STAMPA. - 231:1(2006), pp. 65-73. [10.1016/j.canlet.2005.01.027]
L., Carraresi; R., Martinelli; A., Vannoni; Riccio, Massimo; Dembic, Maja; S., Tripodi; M., Cintorino; S., Santi; E., Bigliardi; M., Carmellini; M., R...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/595903
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