Regulation of brain-derived neurotrophic factor (BDNF) secretion plays a critical role in long-term potentiation (LTP). It is generally thought that the supply for this secretion is newly synthesized BDNF targeted to the synapse. Here we provide evidence that hippocampal neurons additionally recycle BDNF for activity-dependent secretion. Exogenously applied BDNF is internalized by cultured neurons and rapidly becomes available for activity-dependent secretion, which is controlled by the same mechanisms that regulate the secretion of newly synthesized BDNF. Moreover, BDNF recycling replaced the new synthesis pathway in mediating the maintenance of LTP in hippocampal slices: the late phase LTP, which is abolished by protein synthesis inhibition, was rescued in slices preincubated with BDNF. Thus, endocytosed BDNF is fed back to the activity-dependent releasable pool required for LTP maintenance.

Hippocampal neurons recycle BDNF for activity-dependent secretion and LTP maintenance / S., Santi; S., Cappello; Riccio, Massimo; M., Bergami; G., Aicardi; U., Schenk; M., Matteoli; M., Canossa. - In: EMBO JOURNAL. - ISSN 0261-4189. - STAMPA. - 25:18(2006), pp. 4372-4380. [10.1038/sj.emboj.7601303]

Hippocampal neurons recycle BDNF for activity-dependent secretion and LTP maintenance

RICCIO, Massimo;
2006

Abstract

Regulation of brain-derived neurotrophic factor (BDNF) secretion plays a critical role in long-term potentiation (LTP). It is generally thought that the supply for this secretion is newly synthesized BDNF targeted to the synapse. Here we provide evidence that hippocampal neurons additionally recycle BDNF for activity-dependent secretion. Exogenously applied BDNF is internalized by cultured neurons and rapidly becomes available for activity-dependent secretion, which is controlled by the same mechanisms that regulate the secretion of newly synthesized BDNF. Moreover, BDNF recycling replaced the new synthesis pathway in mediating the maintenance of LTP in hippocampal slices: the late phase LTP, which is abolished by protein synthesis inhibition, was rescued in slices preincubated with BDNF. Thus, endocytosed BDNF is fed back to the activity-dependent releasable pool required for LTP maintenance.
2006
25
18
4372
4380
Hippocampal neurons recycle BDNF for activity-dependent secretion and LTP maintenance / S., Santi; S., Cappello; Riccio, Massimo; M., Bergami; G., Aicardi; U., Schenk; M., Matteoli; M., Canossa. - In: EMBO JOURNAL. - ISSN 0261-4189. - STAMPA. - 25:18(2006), pp. 4372-4380. [10.1038/sj.emboj.7601303]
S., Santi; S., Cappello; Riccio, Massimo; M., Bergami; G., Aicardi; U., Schenk; M., Matteoli; M., Canossa
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/595900
Citazioni
  • ???jsp.display-item.citation.pmc??? 40
  • Scopus 100
  • ???jsp.display-item.citation.isi??? 89
social impact