Arylsulphatase activity of Helix pomatia and Patella vulgata have been tested at different temperatures. The maximum pH activity changes depending on temperature; by increasing temperature a shift of pH maximum has been observed in both arylsulphatases. The influence of 4-nitrophenylphosphate (4-NPP), a synthetic inhibitor phosphoester, has been also studied at different temperatures. Differences are evident between inhìbited and non-inhibited arylsulphatase from Helix pomatia and Patella vulgata. By comparing arylsulphatase from Helix and Patella with that of human liver, arylsulphatase from Helix resembles arylsulphatase type A, but it is not inhibited by AgNO3; arylsulphatase from Patella migrates faster than Helix (as human arylsulphatase B does), but is not inhibited by NaCl, inhibitor of arylsulphatase B.
Shift of optimal pH in arylsulphatase from Mollusca depending on temperature / L. C., Sanguini; Pederzoli, Aurora; G., Dubois; M., Masson; E., Davolio; Volpi, Nicola; L., Bolognani. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. B, COMPARATIVE BIOCHEMISTRY. - ISSN 0305-0491. - STAMPA. - 78B (3):(1984), pp. 533-537.
Shift of optimal pH in arylsulphatase from Mollusca depending on temperature
PEDERZOLI, Aurora;VOLPI, Nicola;
1984
Abstract
Arylsulphatase activity of Helix pomatia and Patella vulgata have been tested at different temperatures. The maximum pH activity changes depending on temperature; by increasing temperature a shift of pH maximum has been observed in both arylsulphatases. The influence of 4-nitrophenylphosphate (4-NPP), a synthetic inhibitor phosphoester, has been also studied at different temperatures. Differences are evident between inhìbited and non-inhibited arylsulphatase from Helix pomatia and Patella vulgata. By comparing arylsulphatase from Helix and Patella with that of human liver, arylsulphatase from Helix resembles arylsulphatase type A, but it is not inhibited by AgNO3; arylsulphatase from Patella migrates faster than Helix (as human arylsulphatase B does), but is not inhibited by NaCl, inhibitor of arylsulphatase B.
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