Purifìed heparin, dermatan sulfate and chondroitin sulfate in mixtures were fractionated by anion-exchange chromatography on Ecteola-cellulose using increasing concentration of sodium chloride and ammonium acetate, in thè absence or presence of 10% and 20% methanol, ethanol or propanol. The glycosaminoglycan mixture fractionated with increasing concentration of sodium chloride was recov-ered between 0.2 and 1.6/1.8 M NaCl, with a maximum at about 0.6-0.8 M. Dermatan sulfate was puri-fied from heparin and chondroitin sulfate and was obtained as a single species at 0.2 M NaCl. Anion-exchange chromatography on Ecteola-cellulose with increasing concentration of ammonium acetate demonstrated that glycosaminoglycans were recovered from 0.2 to 2.8/3.0 M. Dermatan sulfate was obtained as a single species at 0.2-0.4 M. The 0.6/0.8 M fractions were composed of different per-centage of dermatan sulfate and chondroitin sulfate. Heparin began to elute at 1.0 M ammonium acetate, and from 1.0 to 1.4 M it was mainly composed of thè fasi moving species. The presence of 10% or 20% methanol, 20% ethanol or 20% propanol had no effect on thè separation of glycosaminogly¬cans, whilst 10% ethanol added to ammonium acetate permitted recovery of large amount dermatan sulfate with a purity of 100% and of heparin with a purity greater than 90%. Moreover, 10% propanol allowed heparin with a purity greater than 95% enriched with fast moving component to be obtained.

Improvement in the fractionation of sulfated glycosaminoglycans by anion-exchange chromatography in the presence of various organic solvents. Preliminary results / Volpi, Nicola. - In: INTERNATIONAL JOURNAL OF BIO-CHROMATOGRAPHY. - ISSN 1068-0659. - STAMPA. - 3 (4):(1997), pp. 245-252.

Improvement in the fractionation of sulfated glycosaminoglycans by anion-exchange chromatography in the presence of various organic solvents. Preliminary results

VOLPI, Nicola
1997

Abstract

Purifìed heparin, dermatan sulfate and chondroitin sulfate in mixtures were fractionated by anion-exchange chromatography on Ecteola-cellulose using increasing concentration of sodium chloride and ammonium acetate, in thè absence or presence of 10% and 20% methanol, ethanol or propanol. The glycosaminoglycan mixture fractionated with increasing concentration of sodium chloride was recov-ered between 0.2 and 1.6/1.8 M NaCl, with a maximum at about 0.6-0.8 M. Dermatan sulfate was puri-fied from heparin and chondroitin sulfate and was obtained as a single species at 0.2 M NaCl. Anion-exchange chromatography on Ecteola-cellulose with increasing concentration of ammonium acetate demonstrated that glycosaminoglycans were recovered from 0.2 to 2.8/3.0 M. Dermatan sulfate was obtained as a single species at 0.2-0.4 M. The 0.6/0.8 M fractions were composed of different per-centage of dermatan sulfate and chondroitin sulfate. Heparin began to elute at 1.0 M ammonium acetate, and from 1.0 to 1.4 M it was mainly composed of thè fasi moving species. The presence of 10% or 20% methanol, 20% ethanol or 20% propanol had no effect on thè separation of glycosaminogly¬cans, whilst 10% ethanol added to ammonium acetate permitted recovery of large amount dermatan sulfate with a purity of 100% and of heparin with a purity greater than 90%. Moreover, 10% propanol allowed heparin with a purity greater than 95% enriched with fast moving component to be obtained.
3 (4)
245
252
Improvement in the fractionation of sulfated glycosaminoglycans by anion-exchange chromatography in the presence of various organic solvents. Preliminary results / Volpi, Nicola. - In: INTERNATIONAL JOURNAL OF BIO-CHROMATOGRAPHY. - ISSN 1068-0659. - STAMPA. - 3 (4):(1997), pp. 245-252.
Volpi, Nicola
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/594791
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