Purifìed heparin, dermatan sulfate and chondroitin sulfate in mixtures were fractionated by anion-exchange chromatography on Ecteola-cellulose using increasing concentration of sodium chloride and ammonium acetate, in thè absence or presence of 10% and 20% methanol, ethanol or propanol. The glycosaminoglycan mixture fractionated with increasing concentration of sodium chloride was recov-ered between 0.2 and 1.6/1.8 M NaCl, with a maximum at about 0.6-0.8 M. Dermatan sulfate was puri-fied from heparin and chondroitin sulfate and was obtained as a single species at 0.2 M NaCl. Anion-exchange chromatography on Ecteola-cellulose with increasing concentration of ammonium acetate demonstrated that glycosaminoglycans were recovered from 0.2 to 2.8/3.0 M. Dermatan sulfate was obtained as a single species at 0.2-0.4 M. The 0.6/0.8 M fractions were composed of different per-centage of dermatan sulfate and chondroitin sulfate. Heparin began to elute at 1.0 M ammonium acetate, and from 1.0 to 1.4 M it was mainly composed of thè fasi moving species. The presence of 10% or 20% methanol, 20% ethanol or 20% propanol had no effect on thè separation of glycosaminogly¬cans, whilst 10% ethanol added to ammonium acetate permitted recovery of large amount dermatan sulfate with a purity of 100% and of heparin with a purity greater than 90%. Moreover, 10% propanol allowed heparin with a purity greater than 95% enriched with fast moving component to be obtained.
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|Anno di pubblicazione:||1997|
|Titolo:||Improvement in the fractionation of sulfated glycosaminoglycans by anion-exchange chromatography in the presence of various organic solvents. Preliminary results|
|Appare nelle tipologie:||Articolo su rivista|
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