Using an optimized and validated ELISA method, we performed serum test for assaying the binding capacity of serum IgG to proteins extracted from approx. 160 different foods to investigate the reactivity of specific IgG antibodies in the Italian population composed of 6879 subjects (4551 females and 2328 males). 44 antigens showed an IgG response greater than 10% and only 14 aliments had an elevated reactivity greater than 20%, in particular, milk, from cow and goat, and several milk derivatives, along with egg albumen and yeasts. The IgG response to the high reactive food antigens depending on the age of the 6880 subjects was also analyzed. We demonstrated a high IgG response in a very large subject group to milk and milk derivatives, and egg albumen antigens, and we conclude that the validated ELISA test may be applied for the serum/plasma IgG antibody level determination as a useful indicator of adverse reactions to food and food hypersensitivity.

Serum IgG responses to food antigens in the Italian population evaluated by highly sensitive and specific ELISA test / Volpi, Nicola; Maccari, Francesca. - In: JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY. - ISSN 1532-1819. - STAMPA. - 30(1):(2009), pp. 51-69.

Serum IgG responses to food antigens in the Italian population evaluated by highly sensitive and specific ELISA test.

VOLPI, Nicola;MACCARI, Francesca
2009

Abstract

Using an optimized and validated ELISA method, we performed serum test for assaying the binding capacity of serum IgG to proteins extracted from approx. 160 different foods to investigate the reactivity of specific IgG antibodies in the Italian population composed of 6879 subjects (4551 females and 2328 males). 44 antigens showed an IgG response greater than 10% and only 14 aliments had an elevated reactivity greater than 20%, in particular, milk, from cow and goat, and several milk derivatives, along with egg albumen and yeasts. The IgG response to the high reactive food antigens depending on the age of the 6880 subjects was also analyzed. We demonstrated a high IgG response in a very large subject group to milk and milk derivatives, and egg albumen antigens, and we conclude that the validated ELISA test may be applied for the serum/plasma IgG antibody level determination as a useful indicator of adverse reactions to food and food hypersensitivity.
30(1)
51
69
Serum IgG responses to food antigens in the Italian population evaluated by highly sensitive and specific ELISA test / Volpi, Nicola; Maccari, Francesca. - In: JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY. - ISSN 1532-1819. - STAMPA. - 30(1):(2009), pp. 51-69.
Volpi, Nicola; Maccari, Francesca
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/594620
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