Toluene diisocyanate (TDI) is the most common cause of occupational asthma in western countries. The aim of this study was to investigate whether genetic factors are involved in toluene diisocyanate-induced asthma. We studied the frequency of human leucocyte antigen (HLA) class II genetic markers in three groups of subjects: 1) subjects with TDI-induced asthma (n=30); 2) exposed subjects with no history of TDI-induced asthma (n=12); and 3) normal subjects not exposed to TDI (n=126). Venous blood samples were collected from the three groups and the polymorphic second exon of DQA and DQB genes was amplified by the polymerase chain reaction (PCR) method. Evaluation of HLA class II gene products in TDI-induced asthma cases showed a positive association with HLA-DQB1*0503 and a negative association with HLADQB1* 0501 alleles, which differed at residue 57 for a single amino acid, i.e. aspartic acid in DQB1*0503 and valine in DQB1*0501. No significant difference was found in the distribution of DQA1 alleles between asthmatics and controls. Our results confirm the hypothesis that HLA-DQB1*0503 has a role in conferring susceptibility to TDI-induced asthma and that residue 57 of HLA-DQB1 is a potentially critical location.

Association between toluene diisocyanate-induced asthma and DQB1 markers: a possible role for aspartic acid at position 57 / A., Balboni; O. R., Baricordi; Fabbri, Leonardo; E., Gandini; A., Ciaccia; C. E., Mapp. - In: EUROPEAN RESPIRATORY JOURNAL. - ISSN 0903-1936. - STAMPA. - 9(1996), pp. 207-210.

Association between toluene diisocyanate-induced asthma and DQB1 markers: a possible role for aspartic acid at position 57.

FABBRI, Leonardo;
1996

Abstract

Toluene diisocyanate (TDI) is the most common cause of occupational asthma in western countries. The aim of this study was to investigate whether genetic factors are involved in toluene diisocyanate-induced asthma. We studied the frequency of human leucocyte antigen (HLA) class II genetic markers in three groups of subjects: 1) subjects with TDI-induced asthma (n=30); 2) exposed subjects with no history of TDI-induced asthma (n=12); and 3) normal subjects not exposed to TDI (n=126). Venous blood samples were collected from the three groups and the polymorphic second exon of DQA and DQB genes was amplified by the polymerase chain reaction (PCR) method. Evaluation of HLA class II gene products in TDI-induced asthma cases showed a positive association with HLA-DQB1*0503 and a negative association with HLADQB1* 0501 alleles, which differed at residue 57 for a single amino acid, i.e. aspartic acid in DQB1*0503 and valine in DQB1*0501. No significant difference was found in the distribution of DQA1 alleles between asthmatics and controls. Our results confirm the hypothesis that HLA-DQB1*0503 has a role in conferring susceptibility to TDI-induced asthma and that residue 57 of HLA-DQB1 is a potentially critical location.
9
207
210
Association between toluene diisocyanate-induced asthma and DQB1 markers: a possible role for aspartic acid at position 57 / A., Balboni; O. R., Baricordi; Fabbri, Leonardo; E., Gandini; A., Ciaccia; C. E., Mapp. - In: EUROPEAN RESPIRATORY JOURNAL. - ISSN 0903-1936. - STAMPA. - 9(1996), pp. 207-210.
A., Balboni; O. R., Baricordi; Fabbri, Leonardo; E., Gandini; A., Ciaccia; C. E., Mapp
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/593748
Citazioni
  • ???jsp.display-item.citation.pmc??? 15
  • Scopus 69
  • ???jsp.display-item.citation.isi??? 61
social impact