The mammalian respiratory tract is densely innervated by sensory and autonomic fibres. Subsets of the nerves contain bioactive regulatory peptides, such as substance P, calcitonin gene-related peptide (CGRP), and neurokinins. The sensory nervous system responds to inhaled irritants, resulting in a release of neuropeptides and, thus, a decrease in the peptide immunoreactivity of the fibres. We examined the effects of inhaled nitrogen dioxide (NO2), a well-known indoor and outdoor air pollutant, on pulmonary sensory neuropeptides. Guinea-pigs were exposed for 4 h to 18 parts per million (ppm) NO2 or to air (n=5 each). At the end of the exposure, they were killed with urethane and their lungs were fixed in 1% paraformaldehyde in phosphate-buffered saline. Cryostat sections were stained with antisera to an anatomical nerve marker, protein gene product (PGP) 9.5, and to CGRP and tachykinins, utilizing the avidin-biotinylated peroxidase method. In the noncartilaginous airways (diameter <250 µm) of NO2-exposed animals, less tachykinin- and CGRP-immunoreactive nerve fibres were found compared with controls. No change was seen in the total nerve fibre distribution (PGP 9.5). It is concluded that the peptidergic nerves of guinea-pig peripheral airways are a sensitive indicator of exposure to nitrogen dioxide.
In vivo exposure to nitrogen dioxide (NO2) induces a decrease in calcitonin gene-related peptide (CGRP) and tachykinin immunoreactivity in guinea-pig peripheral airways / R. E., Lucchini; D. R., Springall; P., Chitano; Fabbri, Leonardo; J. M., Polak; C. E., Mapp. - In: EUROPEAN RESPIRATORY JOURNAL. - ISSN 0903-1936. - STAMPA. - 9(1996), pp. 1847-1851.
Data di pubblicazione: | 1996 |
Titolo: | In vivo exposure to nitrogen dioxide (NO2) induces a decrease in calcitonin gene-related peptide (CGRP) and tachykinin immunoreactivity in guinea-pig peripheral airways. |
Autore/i: | R. E., Lucchini; D. R., Springall; P., Chitano; Fabbri, Leonardo; J. M., Polak; C. E., Mapp |
Autore/i UNIMORE: | |
Rivista: | |
Volume: | 9 |
Pagina iniziale: | 1847 |
Pagina finale: | 1851 |
Codice identificativo ISI: | WOS:A1996VG32800014 |
Codice identificativo Scopus: | 2-s2.0-0029832547 |
Codice identificativo Pubmed: | 8880101 |
Citazione: | In vivo exposure to nitrogen dioxide (NO2) induces a decrease in calcitonin gene-related peptide (CGRP) and tachykinin immunoreactivity in guinea-pig peripheral airways / R. E., Lucchini; D. R., Springall; P., Chitano; Fabbri, Leonardo; J. M., Polak; C. E., Mapp. - In: EUROPEAN RESPIRATORY JOURNAL. - ISSN 0903-1936. - STAMPA. - 9(1996), pp. 1847-1851. |
Tipologia | Articolo su rivista |
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