Functional interactions between adenosine A2A and dopamine D2 receptors have been demonstrated both at the D2 agonist-binding and second messenger levels. The present studies use a [3H]dopamine-binding assay as a sensitive measure of A2A receptor-mediated modulation of D2 receptors. Co-incubation with an A2A receptor agonist increased the Kd value of high-affinity [3H]dopamine-binding sites of the D2 receptor without changing their Bmax values in a cotransfected cell line. This interaction was shown to be subtype specific, as the A2A receptor agonist did not modulate the affinity of the D1 receptor for [3H]dopamine. The domains of the D2 receptor important for the A2A/D2 receptor interaction were studied with chimeric dopamine D2/D1 receptors. The results showed that the A2A receptor agonist still strongly reduced the affinity of a D2/D1 chimera with the sixth transmembrane (TM) domain and third extracellular loop from the D1 receptor. However, the A2A receptor agonist was not able to modulate a D2/D1 chimeric receptor containing the fifth and sixth TM domains and the third intracellular and extracellular loops from the D1 receptor, indicating that the fifth TM domain and/or the third intracellular loop may be involved in the interaction between A2A and D2 receptors.
Biochemical identification of the dopamine D2 receptor domains interacting with the adenosine A2A receptor / M., Torvinen; L. B., Kozell; K. A., Neve; Agnati, Luigi Francesco; K., Fuxe. - In: JOURNAL OF MOLECULAR NEUROSCIENCE. - ISSN 0895-8696. - STAMPA. - 24(2004), pp. 273-280.
Data di pubblicazione: | 2004 |
Titolo: | Biochemical identification of the dopamine D2 receptor domains interacting with the adenosine A2A receptor |
Autore/i: | M., Torvinen; L. B., Kozell; K. A., Neve; Agnati, Luigi Francesco; K., Fuxe |
Autore/i UNIMORE: | |
Rivista: | |
Volume: | 24 |
Pagina iniziale: | 273 |
Pagina finale: | 280 |
Codice identificativo ISI: | WOS:000224628300003 |
Codice identificativo Scopus: | 2-s2.0-12944279339 |
Codice identificativo Pubmed: | 15456930 |
Citazione: | Biochemical identification of the dopamine D2 receptor domains interacting with the adenosine A2A receptor / M., Torvinen; L. B., Kozell; K. A., Neve; Agnati, Luigi Francesco; K., Fuxe. - In: JOURNAL OF MOLECULAR NEUROSCIENCE. - ISSN 0895-8696. - STAMPA. - 24(2004), pp. 273-280. |
Tipologia | Articolo su rivista |
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