E.U.E. (embryonal human explants) cells (general population) were submitted to biochemical and cytoenzymatic tests to compare the enzymatic profile of E.U.E. cells (controls) with that E.U.E. adapted to hypertonic medium. The adapted cells are characterized by very high oxoreductase activity (LDH, HBDH; G-6-P DH) and very high alkaline-phosphatase activity. Clones derived from general population were also submitted to biochemical tests to characterize those more strictly relatedto the enzymatic profile of adapted cells. The profile of clone N. 13 resembles on tis respect that of the adapted cells. The high redox activity is a prerequisite supporting energy supply for osmotic work. The increased activity of plasma membrane enzymes of the adapted cells is also demonstrable in cells exposed for short time to salinity.

Enzymatic characterization of E.U.E. (embryonal human explants) cells adapted to hypertonic media / BOLOGNANI, L; BOLOGNANI FANTIN A., M; CASTELLANA, C; FUHRMAN CONTI A., M; GIULIANI, A; SABATINI, Maria Agnese; SANGUINI C., L; SILVESTRI M., G; TRICOLI, S.. - In: ACTA HISTOCHEMICA. - ISSN 0065-1281. - STAMPA. - 76:(1985), pp. 1-11.

Enzymatic characterization of E.U.E. (embryonal human explants) cells adapted to hypertonic media.

SABATINI, Maria Agnese;
1985

Abstract

E.U.E. (embryonal human explants) cells (general population) were submitted to biochemical and cytoenzymatic tests to compare the enzymatic profile of E.U.E. cells (controls) with that E.U.E. adapted to hypertonic medium. The adapted cells are characterized by very high oxoreductase activity (LDH, HBDH; G-6-P DH) and very high alkaline-phosphatase activity. Clones derived from general population were also submitted to biochemical tests to characterize those more strictly relatedto the enzymatic profile of adapted cells. The profile of clone N. 13 resembles on tis respect that of the adapted cells. The high redox activity is a prerequisite supporting energy supply for osmotic work. The increased activity of plasma membrane enzymes of the adapted cells is also demonstrable in cells exposed for short time to salinity.
1985
76
1
11
Enzymatic characterization of E.U.E. (embryonal human explants) cells adapted to hypertonic media / BOLOGNANI, L; BOLOGNANI FANTIN A., M; CASTELLANA, C; FUHRMAN CONTI A., M; GIULIANI, A; SABATINI, Maria Agnese; SANGUINI C., L; SILVESTRI M., G; TRICOLI, S.. - In: ACTA HISTOCHEMICA. - ISSN 0065-1281. - STAMPA. - 76:(1985), pp. 1-11.
BOLOGNANI, L; BOLOGNANI FANTIN A., M; CASTELLANA, C; FUHRMAN CONTI A., M; GIULIANI, A; SABATINI, Maria Agnese; SANGUINI C., L; SILVESTRI M., G; TRICOL...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/585681
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