Fungal cultures of Microsporum cookei were incubated with aqueous extract of german chamomile [Chamomilla recutita (L.) Rauschert], herniarin (100 and 23 μg/ml) and umbelliferone (100 and 5.5 μg/ml). At the end of the incubation period, the mycelia were irradiated with UVA for 90 min. The fungal morphology was compared with that of control cultures by transmission and scanning electron microscopy. No fungistatic and ultrastructural anomalies were observed for umbelliferone after UVA irradiation. On the contrary herniarin and chamomile extracts show a clear antifungal activity.The main changes were due to inhibition of hyphal outgrowth, apical furcation, alteration of nuclear morphology, deposition of minute electron-dense vesicles in the cytoplasm and thickening of the cell wall. Abnormalities were also involving the mitochondrial feature. These results suggest that the antidermatophytic activity of german chamomile preparations is due to combined effect of herniarin and UVA irradiation.
Antidermatophytic activity of herniarin in Chamomile preparations / Mares, D; Romagnoli, Carlo; Bruni, A.. - In: PLANTES MÉDICINALES ET PHYTOTHÉRAPIE. - ISSN 0032-0994. - STAMPA. - 26:(1993), pp. 91-100.
Antidermatophytic activity of herniarin in Chamomile preparations
ROMAGNOLI, Carlo;
1993
Abstract
Fungal cultures of Microsporum cookei were incubated with aqueous extract of german chamomile [Chamomilla recutita (L.) Rauschert], herniarin (100 and 23 μg/ml) and umbelliferone (100 and 5.5 μg/ml). At the end of the incubation period, the mycelia were irradiated with UVA for 90 min. The fungal morphology was compared with that of control cultures by transmission and scanning electron microscopy. No fungistatic and ultrastructural anomalies were observed for umbelliferone after UVA irradiation. On the contrary herniarin and chamomile extracts show a clear antifungal activity.The main changes were due to inhibition of hyphal outgrowth, apical furcation, alteration of nuclear morphology, deposition of minute electron-dense vesicles in the cytoplasm and thickening of the cell wall. Abnormalities were also involving the mitochondrial feature. These results suggest that the antidermatophytic activity of german chamomile preparations is due to combined effect of herniarin and UVA irradiation.
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