Novel enantioselective silica-supported strong and weak cation exchange (SCX and WCX) materials (3.5 μm particles) based on enantiomerically pure N-(4-allyloxy-3,5-dichlorobenzoyl)-2-amino-3,3-dimethylbutanesulfonic acid and corresponding phosphonic acid as well as carboxylic acid structural analogs as chiral selectors have been evaluated for enantiomer separation of chiral bases by non-aqueous capillary electrochromatography (CEC). Capillary columns packed with these chiral stationary phases (CSPs) showed enantioselectivity in non-aqueous CEC towards a variety of chiral bases including amino alcohols such as β-sympathomimetics and β-blockers. Chromatographic and electrokinetic properties of the strong and weak chiral cation exchangers were evaluated comparatively in terms of their pH* profile, i.e. in terms of their dependence on the base-to-acid ratio of the background electrolyte. It turned out that the SCX type CSPs, and in particular the one based on the β-amino sulfonic acid show a broader window of applicable and suitable experimental conditions for CEC. For example, a strong and constant EOF was obtained on the sulfonic acid based CSP over the entire pH* range studied, while the EOF velocity of the carboxylic acid based CSP was slow under acidic conditions. In the separation of chiral bases, the ion-exchange retention mechanism dominated over electrophoretic migration under most conditions, especially on the SCX type CSPs. The SCX phases exhibited reasonable enantioselectivity over a wider pH* range, while the weak chiral cation exchanger (WCX type CSP) showed enantiomer separation capabilities for primary, secondary, and tertiary chiral amines only in the alkaline pH* range. Sulfonic and phosphonic acid based CSPs possess broad spectrum of applicability. For example, clenbuterol enantiomers were well baseline resolved both on sulfonic acid based CSP (α = 1.33, RS = 14.2) as well as phosphonic acid based CSP (α = 1.13, RS = 4.9). In contrast, under the same conditions the corresponding carboxylic acid CSP exhibited enantioselectivity α of 1.08 and resolution RS of 1.3 only.
Strong versus Weak Chiral Cation Exchangers: Comparative Evaluation for Enantiomer Separation of Chiral Bases by Nonaqueous CEC / E., Zarbl; M., Lämmerhofer; A., Woschek; F., Hammerschmidt; Parenti, Carlo; Cannazza, Giuseppe; W., Lindner. - In: JOURNAL OF SEPARATION SCIENCE. - ISSN 1615-9306. - STAMPA. - 25(2002), pp. 1269-1283.
Data di pubblicazione: | 2002 |
Titolo: | Strong versus Weak Chiral Cation Exchangers: Comparative Evaluation for Enantiomer Separation of Chiral Bases by Nonaqueous CEC |
Autore/i: | E., Zarbl; M., Lämmerhofer; A., Woschek; F., Hammerschmidt; Parenti, Carlo; Cannazza, Giuseppe; W., Lindner |
Autore/i UNIMORE: | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/1615-9314(20021101)25:15/17<1269::AID-JSSC1269>3.0.CO;2-R |
Rivista: | |
Volume: | 25 |
Pagina iniziale: | 1269 |
Pagina finale: | 1283 |
Codice identificativo ISI: | WOS:000179959200040 |
Codice identificativo Scopus: | 2-s2.0-0036924631 |
Citazione: | Strong versus Weak Chiral Cation Exchangers: Comparative Evaluation for Enantiomer Separation of Chiral Bases by Nonaqueous CEC / E., Zarbl; M., Lämmerhofer; A., Woschek; F., Hammerschmidt; Parenti, Carlo; Cannazza, Giuseppe; W., Lindner. - In: JOURNAL OF SEPARATION SCIENCE. - ISSN 1615-9306. - STAMPA. - 25(2002), pp. 1269-1283. |
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