Three-dimensional culture systems have been developed to mimic natural interactions among cells and between cells and the extracellular matrix. The “skin equivalent ” is a 3-D co-culture system of fibroblasts and keratinocytes used as a covering surface in extended wounds, and as a model for studying the influence of each cell type on the synthesis of the extracellular matrix. In the present study, a three-dimensional scaffold made of chitosan, glycosaminoglycans and collagen has been colonised by keratinocytes from newborn foreskin an by fibroblasts isolated from donors of different ages. In the skin equivalent, the neo-synthesized connective tissue is characterized by the presence of amorphous elastic fibers, strengthening the usefulness of this three-dimensional model as a powerful tool for investigating connective tissue metabolism in different physiological (i.e. aging) and pathological conditions. Interestingly, when fibroblasts isolated from aged donors were cultured in vitro, even though in the presence of abundant nutrients and growth factors, their old phenotype appeared not to be completely overcome, indicating that aging is the result of a continuous remodelling controlled by epigenetic as well as genetic factors. Finally, the aged phenotype of fibroblasts seems to exert a stronger influence on keratinocytes compared to that of keratinocytes on fibroblasts.

The skin equivalent model: developments and perspectives / Croce, Maria Antonietta; Sammarco, Rita; PAOLINELLI DEVINCENZI, Chiara; Boraldi, Federica; Gheduzzi, Dealba; Damour, O.; Sommer, P.; Ronchetti, Ivonne; Tiozzo, Roberta; Quaglino, Daniela. - In: MICROSCOPIE. - STAMPA. - 2:(2004), pp. 31-36.

The skin equivalent model: developments and perspectives.

CROCE, Maria Antonietta;SAMMARCO, Rita;PAOLINELLI DEVINCENZI, Chiara;BORALDI, Federica;GHEDUZZI, Dealba;RONCHETTI, Ivonne;TIOZZO, Roberta;QUAGLINO, Daniela
2004

Abstract

Three-dimensional culture systems have been developed to mimic natural interactions among cells and between cells and the extracellular matrix. The “skin equivalent ” is a 3-D co-culture system of fibroblasts and keratinocytes used as a covering surface in extended wounds, and as a model for studying the influence of each cell type on the synthesis of the extracellular matrix. In the present study, a three-dimensional scaffold made of chitosan, glycosaminoglycans and collagen has been colonised by keratinocytes from newborn foreskin an by fibroblasts isolated from donors of different ages. In the skin equivalent, the neo-synthesized connective tissue is characterized by the presence of amorphous elastic fibers, strengthening the usefulness of this three-dimensional model as a powerful tool for investigating connective tissue metabolism in different physiological (i.e. aging) and pathological conditions. Interestingly, when fibroblasts isolated from aged donors were cultured in vitro, even though in the presence of abundant nutrients and growth factors, their old phenotype appeared not to be completely overcome, indicating that aging is the result of a continuous remodelling controlled by epigenetic as well as genetic factors. Finally, the aged phenotype of fibroblasts seems to exert a stronger influence on keratinocytes compared to that of keratinocytes on fibroblasts.
2
31
36
The skin equivalent model: developments and perspectives / Croce, Maria Antonietta; Sammarco, Rita; PAOLINELLI DEVINCENZI, Chiara; Boraldi, Federica; Gheduzzi, Dealba; Damour, O.; Sommer, P.; Ronchetti, Ivonne; Tiozzo, Roberta; Quaglino, Daniela. - In: MICROSCOPIE. - STAMPA. - 2:(2004), pp. 31-36.
Croce, Maria Antonietta; Sammarco, Rita; PAOLINELLI DEVINCENZI, Chiara; Boraldi, Federica; Gheduzzi, Dealba; Damour, O.; Sommer, P.; Ronchetti, Ivonne; Tiozzo, Roberta; Quaglino, Daniela
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/466907
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact