This paper describes the properites of a cell-based assay for the detection and quantification of yessotoxin-group toxins contaminating shellfish extracts. The procedure exploits the property of yessotoxin to induce the accumulation of a 100 kDa fragment of E-cadherin in epithelial cells. The dose-dependence of this response is used for quntification of contaminating agents in relevant extracts.
A functional assay for yessotoxins / Pierotti, S.; Ferrari, S.; Malaguti, C.; Milandri, A.; Poletti, R.; Rossini, Gian Paolo. - STAMPA. - Cawthron report No. 906:(2003), pp. 96-101. (Intervento presentato al convegno HABTECH 2003 tenutosi a Nelson nel 26-30 nov. 2003).
A functional assay for yessotoxins
ROSSINI, Gian Paolo
2003
Abstract
This paper describes the properites of a cell-based assay for the detection and quantification of yessotoxin-group toxins contaminating shellfish extracts. The procedure exploits the property of yessotoxin to induce the accumulation of a 100 kDa fragment of E-cadherin in epithelial cells. The dose-dependence of this response is used for quntification of contaminating agents in relevant extracts.
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