3T3 cells null for the type 1 insulin-like growth factor receptor are refractory to stimulation by a variety of purified growth factors that are known to be required for the stimulation of other 3T3 cells. However, these cells, known as R- cells, grow in serum-supplemented medium and also in media conditioned by certain cell lines. We report here the purification of a growth factor that stimulates DNA synthesis (and growth) of R- cells. The growth factor, purified to homogeneity by SDS-polyacrylamide gel electrophoresis, was identified as the granulin/epithelin precursor by an accurate determination of the masses of endoproteinase Lys-C peptides using matrix-assisted laser desorption ionization mass spectrometry, followed by a data base search. The granulin/epithelin precursor is a little known growth factor, secreted by a variety of epithelial and hemopoietic cells. It is at present the only purified growth factor that can stimulate the growth of mouse embryo fibroblasts null for the type 1 insulin-like growth factor receptor.
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http://hdl.handle.net/11380/460679
Anno di pubblicazione: | 1998 |
Titolo: | The Granulin/Epithelin Precursor Abrogates the Requirement for the Insulin-like Growth Factor 1 Receptor for Growth in Vitro |
Autori: | XU; S-Q; TANG; D; CHAMBERLAIN; S; PRONK; G; MASIARZ; F. R; KAUR; S; PRISCO; M; T. ZANOCCO MARANI; T; AND BASERGA; R |
Autori: | |
Rivista: | |
Volume: | 273(51) |
Pagina iniziale: | 34661 |
Pagina finale: | 34670 |
Appare nelle tipologie: | Articolo su rivista |
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