Several genes encoding subunits of the neuronal nicotinic acetylcholine receptors have been cloned and regulatory elements involved in the transcription of the alpha 2 and alpha 7-subunit genes have been described. Yet, the detailed mechanisms governing the neuron-specific transcription and the spatio-temporal expression pattern of these genes remain largely uninvestigated. The beta 2-subunit is the most widely expressed neuronal nicotinic receptor subunit in the nervous system. We have studied the structural and regulatory properties of the 5' sequence of this gene. A fragment of 1163 bp of upstream sequence is sufficient to drive the cell-specific transcription of a reporter gene in both transient transfection assays and in transgenic mice. Deletion analysis and site-directed mutagenesis of this promoter reveal two negative elements and one positive element. The positively-acting sequence includes one functional E-box. One of the repressor elements is located in the transcribed region and is the NRSE/RE1 sequence already described in promoters of neuronal genes. In this paper, we describe the neuron-specific promoter of the gene encoding the neuronal nicotinic acetylcholine receptor beta 2-subunit.

Promoter elements conferring neuron specific expression of the b2 subunit of the neuronal nicotinic acetylcholine receptor studied in vitro and in transgenic mice / Bessis, A.; Salmon, A. M.; Zoli, Michele; LE NOVERE, N.; Picciotto, M. R.; Changeux, J. P.. - In: NEUROSCIENCE. - ISSN 0306-4522. - STAMPA. - 69:(1995), pp. 807-819.

Promoter elements conferring neuron specific expression of the b2 subunit of the neuronal nicotinic acetylcholine receptor studied in vitro and in transgenic mice.

ZOLI, Michele;
1995

Abstract

Several genes encoding subunits of the neuronal nicotinic acetylcholine receptors have been cloned and regulatory elements involved in the transcription of the alpha 2 and alpha 7-subunit genes have been described. Yet, the detailed mechanisms governing the neuron-specific transcription and the spatio-temporal expression pattern of these genes remain largely uninvestigated. The beta 2-subunit is the most widely expressed neuronal nicotinic receptor subunit in the nervous system. We have studied the structural and regulatory properties of the 5' sequence of this gene. A fragment of 1163 bp of upstream sequence is sufficient to drive the cell-specific transcription of a reporter gene in both transient transfection assays and in transgenic mice. Deletion analysis and site-directed mutagenesis of this promoter reveal two negative elements and one positive element. The positively-acting sequence includes one functional E-box. One of the repressor elements is located in the transcribed region and is the NRSE/RE1 sequence already described in promoters of neuronal genes. In this paper, we describe the neuron-specific promoter of the gene encoding the neuronal nicotinic acetylcholine receptor beta 2-subunit.
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Promoter elements conferring neuron specific expression of the b2 subunit of the neuronal nicotinic acetylcholine receptor studied in vitro and in transgenic mice / Bessis, A.; Salmon, A. M.; Zoli, Michele; LE NOVERE, N.; Picciotto, M. R.; Changeux, J. P.. - In: NEUROSCIENCE. - ISSN 0306-4522. - STAMPA. - 69:(1995), pp. 807-819.
Bessis, A.; Salmon, A. M.; Zoli, Michele; LE NOVERE, N.; Picciotto, M. R.; Changeux, J. P.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/460658
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