Nicotine affects many aspects of behaviour including learning and memory through its interaction with neuronal nicotinic acetylcholine receptors (nAChR). Functional nAChRs are pentameric proteins containing at least one type of alpha-subunit and one type of beta-subunit. The involvement of a particular neuronal nicotinic subunit in pharmacology and behaviour was examined using gene targeting to mutate beta 2, the most widely expressed nAChR subunit in the central nervous system. We report here that high-affinity binding sites for nicotine are absent from the brains of mice homozygous for the beta 2-subunit mutation. Further, electrophysiological recording from brain slices reveals that thalamic neurons from these mice do not respond to nicotine application. Finally, behavioural tests demonstrate that nicotine no longer augments the performance of beta 2-1- mice on passive avoidance, a test of associative memory. Paradoxically, mutant mice are able to perform better than their non-mutant siblings on this task.

Abnormal avoidance learning in mice lacking functional high-affinity nicotine receptor in the brain / Picciotto, Mr; Zoli, Michele; Lena, C; Bessis, A; Lallemand, Y; LE NOVERE, N; Vincent, P; MERLO PICH, E; Brulet, P; Changeux, Jp. - In: NATURE. - ISSN 0028-0836. - STAMPA. - 374(1995), pp. 65-67.

Abnormal avoidance learning in mice lacking functional high-affinity nicotine receptor in the brain

ZOLI, Michele;
1995

Abstract

Nicotine affects many aspects of behaviour including learning and memory through its interaction with neuronal nicotinic acetylcholine receptors (nAChR). Functional nAChRs are pentameric proteins containing at least one type of alpha-subunit and one type of beta-subunit. The involvement of a particular neuronal nicotinic subunit in pharmacology and behaviour was examined using gene targeting to mutate beta 2, the most widely expressed nAChR subunit in the central nervous system. We report here that high-affinity binding sites for nicotine are absent from the brains of mice homozygous for the beta 2-subunit mutation. Further, electrophysiological recording from brain slices reveals that thalamic neurons from these mice do not respond to nicotine application. Finally, behavioural tests demonstrate that nicotine no longer augments the performance of beta 2-1- mice on passive avoidance, a test of associative memory. Paradoxically, mutant mice are able to perform better than their non-mutant siblings on this task.
374
65
67
Abnormal avoidance learning in mice lacking functional high-affinity nicotine receptor in the brain / Picciotto, Mr; Zoli, Michele; Lena, C; Bessis, A; Lallemand, Y; LE NOVERE, N; Vincent, P; MERLO PICH, E; Brulet, P; Changeux, Jp. - In: NATURE. - ISSN 0028-0836. - STAMPA. - 374(1995), pp. 65-67.
Picciotto, Mr; Zoli, Michele; Lena, C; Bessis, A; Lallemand, Y; LE NOVERE, N; Vincent, P; MERLO PICH, E; Brulet, P; Changeux, Jp
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/460626
Citazioni
  • ???jsp.display-item.citation.pmc??? 212
  • Scopus 559
  • ???jsp.display-item.citation.isi??? 537
social impact